Analysis of the bovine neutrophil transcriptome during glucocorticoid treatment
| Autor: | Sally A. Madsen-Bouterse, Robert G. Halgren, Xiaoning Ren, Jeanne L. Burton, Sue Sipkovsky, Jennifer L Barrick, Rachael Kruska, Patty S.D. Weber, Guilherme J. M. Rosa, Patricia E. Almeida |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Transcription Genetic Physiology Neutrophils Total rna Apoptosis Biology Dexamethasone Translocation Genetic Transcriptome Receptors Glucocorticoid polycyclic compounds Genetics medicine Animals RNA Messenger Glucocorticoids Progesterone Polymorphonuclear neutrophil Gene Expression Profiling Flow Cytometry Extracellular Matrix Mifepristone Phenotype Immunology Cattle hormones hormone substitutes and hormone antagonists Glucocorticoid medicine.drug |
| Zdroj: | Physiological genomics. 28(1) |
| ISSN: | 1531-2267 |
| Popis: | The objective of this study was to characterize a large portion of the bovine neutrophil transcriptome following treatment with the anti-inflammatory glucocorticoid dexamethasone (Dex). Total RNA was isolated from blood neutrophils of healthy cattle (5 castrated male Holsteins) immediately following cell purification (0 h) or after ex vivo aging for 4 h with or without added Dex. Additional neutrophils were cotreated with a glucocorticoid receptor (GR) antagonist (RU486) and Dex for 4 h. RNA was amplified, dye labeled (Cy3 or Cy5), and hybridized to a series of National Bovine Functional Genomics Consortium (NBFGC) microarrays. LOWESS data normalization followed by mixture model analyses showed that 11.15% of the spotted NBFGC cDNAs (2,036/18,263) were expressed in 4-h (untreated) neutrophils. Subsequent two-step mixed-model analysis detected ( P ≤ 0.05) 1,109 differentially expressed genes, of which contrast analysis indicated those that were independently responsive to aging (1,064), Dex (502), RU486 + Dex (141), or RU486 (357). In silico analysis revealed that 416 of the differentially expressed genes are unknown, 59 did not cluster well based on known function, and 634 clustered into 20 ontological categories. Independent validation of differential expression was done for 14 of the putatively Dex-responsive genes across these categories. Results showed that Dex induced rapid translocation of GR into the neutrophil nucleus and signaled dramatic alterations in expression of genes that delay apoptosis, enhance bactericidal activity, and promote tissue remodeling without inflammation or fibrosis. Thus these findings revealed hitherto unappreciated plasticity of blood neutrophils and potentially novel anti-inflammatory/wound-healing actions of glucocorticoids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|