Characterization of a Biofilm Bioreactor Designed for the Single-Step Production of Aerial Conidia and Oosporein by Beauveria bassiana PQ2
| Autor: | Juan A. Ascacio-Valdés, José Guadalupe Ávila-Hernández, Fabiola Veana, Diana B. Muñiz-Márquez, Jorge E. Wong-Paz, Luis V. Rodríguez-Durán, Pedro Aguilar-Zárate, Mariela Ramona Michel, Mayra Aguilar-Zárate, Héctor Raziel Lara-Juache |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) QH301-705.5 030106 microbiology Beauveria bassiana biological control Plant Science Bassiana 01 natural sciences Conidium 03 medical and health sciences 010608 biotechnology spore production Bioreactor Food science Biology (General) Ecology Evolution Behavior and Systematics oosporein biology Chemistry fungi biology.organism_classification Spore Entomopathogenic fungus Fermentation Aeration |
| Zdroj: | Journal of Fungi, Vol 7, Iss 582, p 582 (2021) Journal of Fungi Volume 7 Issue 8 |
| Popis: | Beauveria bassiana is an entomopathogenic fungus that is used for the biological control of different agricultural pest insects. B. bassiana is traditionally cultivated in submerged fermentation and solid-state fermentation systems to obtain secondary metabolites with antifungal activity and infective spores. This work presents the design and characterization of a new laboratory-scale biofilm bioreactor for the simultaneous production of oosporein and aerial conidia by B. bassiana PQ2. The reactor was built with materials available in a conventional laboratory. KLa was determined at different air flows (1.5–2.5 L/min) by two different methods in the liquid phase and in the exhaust gases. The obtained values showed that an air flow of 2.5 L/min is sufficient to ensure adequate aeration to produce aerial conidia and secondary metabolites by B. bassiana. Under the conditions studied, a concentration of 183 mg oosporein per liter and 1.24 × 109 spores per gram of support was obtained at 168 h of culture. These results indicate that the biofilm bioreactor represents a viable alternative for the production of products for biological control from B. bassiana. |
| Databáze: | OpenAIRE |
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