B7-H3 Over Expression in Prostate Cancer Promotes Tumor Cell Progression
| Autor: | Hexing Yuan, Guangbo Zhang, Xuedong Wei, Jianquan Hou, Chen Li, Xueguang Zhang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
PCA3 Pathology medicine.medical_specialty B7 Antigens Urology Cell Prostatic Hyperplasia Prostate cancer Antigens CD Prostate medicine Humans Receptors Immunologic Cell adhesion Aged Aged 80 and over Cell growth business.industry Prostatic Neoplasms Middle Aged Hyperplasia medicine.disease Gene Expression Regulation Neoplastic Prostate-specific antigen medicine.anatomical_structure Disease Progression Cancer research business |
| Zdroj: | Journal of Urology. 186:1093-1099 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/j.juro.2011.04.103 |
| Popis: | We compared B7-H3 expression in benign prostatic hyperplasia and prostate cancer tissue specimens, and determined the effects of low B7-H3 expression on the PC-3 human prostate cancer cell line using RNA interference.B7-H3 expression in prostate specimens was determined by enzyme-linked immunosorbent assay. A PC-3 cell line with low B7-H3 expression was established by RNA interference to investigate the effect of B7-H3 on cell proliferation, adhesion, migration and invasion in vitro.B7-H3 in tissue samples was significantly higher in the prostate cancer group than in the benign prostatic hyperplasia group (mean±SEM 174.73±56.80 vs 82.69±46.19 ng/gm, p0.001). B7-H3 expression down-regulated by small interfering RNA decreased cell adhesion to PC-3 fibronectin more than 30%, and migration and Matrigel™ invasion up to 50%. No apparent impact was observed on cell proliferation.B7-H3 is aberrantly expressed in prostate cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating PC-3 cell progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|