Profiling proteins in nutraceutical formulations: characterization of the constituents
| Autor: | Ridvan Nepravishta, Maurizio Paci, Mario Marchetti, Alessia Bellomaria |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Analytical chemistry 010402 general chemistry 01 natural sciences Analytical Chemistry 03 medical and health sciences Nutraceutical Animals Spectroscopy chemistry.chemical_classification 030109 nutrition & dietetics Chromatography Chemistry Proteins General Medicine Nuclear magnetic resonance spectroscopy Small molecule NMR 0104 chemical sciences Amino acid Protein profiling Milk whey proteins Electrophoresis Milk Whey Proteins Dietary Supplements Proton NMR Settore CHIM/10 - Chimica degli Alimenti Food Science |
| Zdroj: | Food chemistry. 194 |
| ISSN: | 1873-7072 |
| Popis: | Several nutraceutical preparations containing proteins, amino acids and other small molecules are nowadays present on the market. In this work we propose NMR spectroscopy such as (1)H NMR, (1)H-(1)H TOCSY and DOSY for their constituents characterization, identification and profiling, comparing these results with those obtained by electrophoretic technique such as SDS-PAGE. The (1)H NMR spectroscopy was applied for measurements of the amino acids and other small compounds added from the manufacturer. Further the autocorrelation function obtained from the one dimensional spectrum was used without the complete assignment of the resonances of the NMR spectrum of proteins for the evaluation of the folding quality and stability. Finally the DOSY NMR technique was performed on the samples for the characterization of the mean molecular weight range of proteins. All this features considered together create an important set of data useful for the evaluation of the protein profiling and the characterization of such formulations. |
| Databáze: | OpenAIRE |
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