Short communication: Passive shedding of Mycobacterium avium ssp. paratuberculosis in commercial dairy goats in Brazil
| Autor: | Leandro Licursi de Oliveira, Magna Coroa Lima, Marcus Vinícius de Andrade Barros, Thalita Moreira Scatamburlo, Maria Aparecida Scatamburlo Moreira, D. G. G. Schwarz, Fabrício Luciani Valente, C.T.S.A.M. Oliveira, N. Rosado |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Veterinary medicine
medicine.medical_specialty 040301 veterinary sciences Paratuberculosis Enteritis 0403 veterinary science Feces Animal science Passive contamination Genetics medicine Animals Bacterial Shedding Goat Diseases biology Goats 0402 animal and dairy science 04 agricultural and veterinary sciences medicine.disease biology.organism_classification 040201 dairy & animal science Mycobacterium avium subsp. paratuberculosis Goat farming Milk Goat Animal Science and Zoology Histopathology Brazil Food Science Mycobacterium |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| Popis: | Goat farming is a low-cost alternative to dairy production in developing countries. In Brazil, goat production has increased in recent years due in part to the implementation of programs encouraging this activity. Mycobacterium avium ssp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a disease that causes chronic granulomatous enteritis in ruminants, but MAP transmission dynamics are still poorly understood in goats. In a previously published study of our research group, 10 dairy goat farms (467 animals) from Minas Gerais state were analyzed for MAP detection; 2 fecal cultures and 11 milk samples tested positive for MAP by conventional PCR and were confirmed by sequencing. Because no clinical signs were observed over 1 yr of monitoring, we hypothesized that these MAP-positive goats could be passive shedders. Thus, in the present study, 4 positive goats (4/13) from the previous study were purchased and feces and milk samples were collected for evaluation (twice, with an interval of 3 mo between tests) by culture of MAP, IS900 PCR, or both. All analyses were negative for MAP. At the last time point, blood samples were collected for ELISA, the animals were killed, and tissues collected for tissue culture and histopathology. At necropsy, no macroscopic lesions related to paratuberculosis were observed. Similarly, no histological changes were observed and MAP in samples stained by Ziehl-Neelsen was not detected. These animals were characterized as potential passive shedders with upward contamination of the teat canal by MAP. This is the first report of the passive shedding phenomenon in goats in Brazil and it highlights the importance of identifying these animals for control programs and to ensure the quality of dairy products. |
| Databáze: | OpenAIRE |
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