Bone marrow stem cells accelerate lung maturation and prevent the LPS-induced delay of morphological and functional fetal lung development in the presence of ErbB4
| Autor: | Kyra Bokel, Katja Zscheppang, Lavinia Ionescu, Verena Huhn, Christiane E.L. Dammann, Andreas Schmiedl |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Receptor ErbB-4 Histology Organogenesis Mesenchyme Mice Transgenic Lung injury Mesenchymal Stem Cell Transplantation Pathology and Forensic Medicine Fetal Development Andrology Mice 03 medical and health sciences Fetus 0302 clinical medicine medicine Animals Lung business.industry Mesenchymal stem cell Bone Marrow Stem Cell Mesenchymal Stem Cells Cell Biology Haematopoiesis 030104 developmental biology medicine.anatomical_structure Female Stem cell business 030217 neurology & neurosurgery |
| Zdroj: | Cell and Tissue Research. 380:547-564 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | ErbB4 is a regulator in lung development and disease. Prenatal infection is an important risk factor for the delay of morphologic lung development, while promoting the maturation of the surfactant system. Bone marrow–derived mesenchymal stem cells (BMSCs) have the potential to prevent lung injury. We hypothesized that BMSCs in comparison with hematopoietic control stem cells (HPSCs) minimize the lipopolysaccharide (LPS)-induced lung injury only when functional ErbB4 receptor is present. We injected LPS and/or murine green fluorescent protein–labeled BMSCs or HPSCs into the amniotic cavity of transgenic ErbB4heart mothers at gestational day 17. Fetal lungs were analyzed 24 h later. BMSCs minimized significantly LPS-induced delay in morphological lung maturation consisting of a stereologically measured increase in mesenchyme and septal thickness and a decrease of future airspace and septal surface. This effect was more prominent and significant in the ErbB4heart+/− lungs, suggesting that the presence of functioning ErbB4 signaling is required. BMSC also diminished the LPS induced increase in surfactant protein (Sftp)a mRNA and decrease in Sftpc mRNA is only seen if ErbB4 is present. The reduction of morphological delay of lung development and of levels of immune-modulating Sftp was more pronounced in the presence of the ErbB4 receptor. Thus, ErbB4 may be required for the protective signaling of BMSCs. |
| Databáze: | OpenAIRE |
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