Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs
| Autor: | Chunying Shi, Huili Wang, Yue Lin, Xin Yan, Liangyun Shen, Qian Tan, Jianwu Dai, Shuqin Wang, Shiyan Li, Xiang Lin Hou, Yun Yang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Scaffold
medicine.medical_specialty mesenchymal stem cells affinity peptide Cell Biomedical Engineering lcsh:Medicine Peptide Dermatology Critical Care and Intensive Care Medicine Collagen-binding domain In vivo medicine Immunology and Allergy chemistry.chemical_classification business.industry Regeneration (biology) Mesenchymal stem cell lcsh:R Adhesion Surgery Cell biology medicine.anatomical_structure chemistry skin wound healing Emergency Medicine Mesenchymal stem cells Original Article business Wound healing |
| Zdroj: | Burns & Trauma, Vol 2, Iss 4, Pp 181-186 (2014) Burns & Trauma |
| ISSN: | 2321-3868 |
| DOI: | 10.4103/2321-3868.143623 |
| Popis: | Mesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs (E7 peptide) was immobilized to a collagen scaffold via a collagen-binding domain (CBD) to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. In vivo investigations indicated that due to the immunoreaction, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen/CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen/CBD-E7 peptide group, with significant differences compared with the other groups (P < 0.05, P < 0.01). The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds. |
| Databáze: | OpenAIRE |
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