Ultrastructural and Electron Energy Loss Spectroscopy Studies of Sequestration Mechanisms of Cd and Cu in the Marine Diatom Skeletonema costatum
| Autor: | Jean-Claude Amiard, J. L. Mansot, T. Ginsburger-Vogel, Jany Wéry, Y. Nassiri |
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| Rok vydání: | 1997 |
| Předmět: |
Diatoms
inorganic chemicals Cadmium Chemistry Spectrophotometry Atomic Health Toxicology and Mutagenesis chemistry.chemical_element General Medicine Vacuole Toxicology Pollution Nitrogen Sulfur Copper Metal Microscopy Electron visual_art Environmental chemistry Botany visual_art.visual_art_medium Ecotoxicology Phytotoxicity |
| Zdroj: | Archives of Environmental Contamination and Toxicology. 33:147-155 |
| ISSN: | 1432-0703 0090-4341 |
| DOI: | 10.1007/s002449900236 |
| Popis: | The marine diatom Skeletonema costatum was used to study mechanisms of detoxification when submitted to cadmium and copper contamination. After 96 h of growth, concentration corresponding to 50% growth inhibition (IC50, 96 h) was 0.224 mg/L for cadmium and 0.045 mg/L for copper, indicating that copper is more toxic for S. costatum than cadmium. Heavy cellular damages were observed for cadmium and copper concentrations close to the IC50. Exposure to these concentrations induced a migration of inclusions from the peripheral cytoplasm to the vacuole. Electron energy loss spectroscopy (EELS) investigations demonstrated that Cd and Cu were specifically trapped in these inclusions. However, Cu was less sequestered than cadmium in the vacuole. EELS determination of oxidation states evidenced that trace metals were sequestered as Cd2+ and Cu2+. Nitrogen and sulfur are involved in metallic storage, especially in the case of cadmium contamination. |
| Databáze: | OpenAIRE |
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