Detection of Autoantibodies to the Pancreatic Islet Heat Shock Protein 60 in Insulin-dependent Diabetes Mellitus
| Autor: | Akira Shimada, Tomohiro Kasatani, Yukako Ozawa, Akira Kasuga, Taro Maruyama, Hiroko Nomaguchi, Takao Saruta, Izumi Takei, Junichi Miyazaki |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
endocrine system medicine.medical_specialty animal structures endocrine system diseases Immunology Islets of Langerhans Immunopathology Internal medicine Diabetes mellitus medicine Humans Immunology and Allergy Fluorescent Antibody Technique Indirect Autoantibodies Autoimmune disease geography geography.geographical_feature_category biology business.industry fungi Autoantibody nutritional and metabolic diseases Chaperonin 60 Middle Aged medicine.disease Islet Diabetes Mellitus Type 1 Endocrinology Rheumatoid arthritis biology.protein Female HSP60 Antibody business |
| Zdroj: | Journal of Autoimmunity. 9:517-524 |
| ISSN: | 0896-8411 |
| DOI: | 10.1006/jaut.1996.0069 |
| Popis: | Autoantibodies against heat shock protein (hsp) 60 have been reported to be detected in sera of non-obese diabetic mice, in an experimental model of IDDM. However, there are only a few studies which have examined IDDM patients for antibodies against mammalian hsp60. We produced murine hsp60 derived from pancreatic beta cells which has high homology to human hsp60 and examined antibodies against the hsp60 in IDDM patients using an enzyme-linked immunosorbent assay. We extended the analysis to patients with other immune-mediated diseases and non-insulin-dependent diabetes mellitus (NIDDM). Positive sera for hsp60 antibody were more frequently detected in 13 out of 84 IDDM (15.5%) and 5 out of 25 rheumatoid arthritis patients (20%), when compared to healthy subjects (1/85; 1.2%, P0.001 and P0.01, respectively). The levels of hsp60 antibodies of IDDM (0.218 +/- 0.227) and rheumatoid arthritis patients (0.259 +/- 0.191) were significantly higher than those of healthy subjects (0.076 +/- 0.131, P0.001, P0.01, respectively). Patients with slowly progressive IDDM (n = 26), autoimmune thyroid disease (n = 42), or NIDDM (n = 40) had levels of hsp60 antibodies similar to those in healthy subjects. We found no relationship between the levels of hsp60 antibodies and islet cell antibodies (ICA) or antibodies to glutamic acid decarboxylase (GAD65) in IDDM patients. In conclusion, hsp60 antibodies were detected in Japanese IDDM as well as in rheumatoid arthritis patients. Although the positivity was low, the detection of hsp60 antibodies may be helpful for diagnosis of IDDM especially in GAD65 Ab- or JCA-negative Japanese patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect