The Envelope Glycoprotein of Simian Immunodeficiency Virus Contains an Enterotoxin Domain
| Autor: | Mark J McArthur, V. W. Cox, Andrey Frolov, Richard W. Compans, Suxiang Tong, Judith M. Ball, C. L. Swaggerty |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Diarrhea
envelope glycoprotein chemistry.chemical_element Enterotoxin Inositol 1 4 5-Trisphosphate Calcium Biology medicine.disease_cause Calcium in biology Microbiology Cell Line chemistry.chemical_compound Enterotoxins Mice Intestinal mucosa Chlorides Viral Envelope Proteins Rotavirus Virology medicine Animals Humans Inositol Intestinal Mucosa Glycoproteins chemistry.chemical_classification Mice Inbred BALB C Dose-Response Relationship Drug Simian immunodeficiency virus chloride secretion Animals Suckling Intestines calcium mobilization SIV chemistry Simian Immunodeficiency Virus Glycoprotein HT29 Cells viral enterotoxin |
| Zdroj: | Virology. 277(2):250-261 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2000.0626 |
| Popis: | By the use of a mouse model, the enteropathic effects of the simian immunodeficiency virus (SIV) surface unit (SU) envelope glycoprotein were explored. Purified SU (0.01–0.45 nmol) was administered intraperitoneally to 6- to 8-day-old mouse pups and induced a dose-dependent diarrheal response. Surgical introduction of SU into adult mouse intestinal loops revealed fluid accumulation without histological alterations and SU-treated unstripped intestinal mucosa induced chloride (Cl − ) secretory currents in Ussing chambers. Similarly to rotavirus NSP4, the first described viral enterotoxin, SU induced a transient increase in intracellular calcium levels and increased inositol 1,4,5-triphosphate (IP 3 ) levels in HT-29 cells. These data indicate the calcium response is mediated by IP 3 . The presence of diarrhea and fluid accumulation within intestinal loops in the absence of histological alterations and induction of Cl − secretory currents demonstrate that SIV contains an enterotoxic domain localized within SU and is the second viral enterotoxin described. |
| Databáze: | OpenAIRE |
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