The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Autor: Emilie Jolly, Laurence Mazaux, Benoit Visseaux, Tiffany Pascreau, Philippe Lesprit, Marianne Asso-Bonnet, Nadira Houhou, Eric Farfour, Marc Vasse
Rok vydání: 2020
Předmět:
Microbiology (medical)
medicine.medical_specialty
Coronavirus disease 2019 (COVID-19)
viruses
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Pneumonia
Viral
RNA-dependent RNA polymerase
Viral Nonstructural Proteins
Betacoronavirus
Coronavirus Envelope Proteins
COVID-19 Testing
Medical microbiology
Viral Envelope Proteins
Nasopharynx
medicine
Coronavirus Nucleocapsid Proteins
Humans
Prospective Studies
Pandemics
Gene
Coronavirus RNA-Dependent RNA Polymerase
biology
Clinical Laboratory Techniques
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
COVID-19
General Medicine
Infection diagnosis
Nucleocapsid Proteins
Phosphoproteins
RNA-Dependent RNA Polymerase
biology.organism_classification
Virology
Infectious Diseases
Real-time polymerase chain reaction
France
Coronavirus Infections
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases
ISSN: 1435-4373
0934-9723
Popis: Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets.
Databáze: OpenAIRE
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