Life-Long Neural Stem Cells Are Fate-Specified at an Early Developmental Stage
| Autor: | Aoi Tanaka, Takahiro Fuchigami, Seiji Hitoshi, Shohei Ishida, Anri Kuroda, Yoshitaka Hayashi, Yugo Fukazawa, Kazuhiro Ikenaka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cognitive Neuroscience
Population Genetic Vectors Mice Transgenic Biology Cellular and Molecular Neuroscience Neural Stem Cells lentivirus Precursor cell Neurosphere Cortex (anatomy) medicine Animals Cell Lineage education reproductive and urinary physiology education.field_of_study Early embryonic stage clonal analysis Brain Embryonic stem cell barcoding Neural stem cell Olfactory bulb Cell biology nervous system diseases medicine.anatomical_structure nervous system mouse neural stem cells biological phenomena cell phenomena and immunity neurospheres |
| Zdroj: | Cerebral cortex (New York, N.Y. : 1991). 30(12):6415-6425 |
| ISSN: | 1460-2199 |
| Popis: | The origin and life-long fate of quiescent neural stem cells (NSCs) in the adult mammalian brain remain largely unknown. A few neural precursor cells in the embryonic brain elongate their cell cycle time and subsequently become quiescent postnatally, suggesting the possibility that life-long NSCs are selected at an early embryonic stage. Here, we utilized a GFP-expressing lentivirus to investigate the fate of progeny from individual lentivirus-infected NSCs by identifying the lentiviral integration site. Our data suggest that NSCs become specified to two or more lineages prior to embryonic day 13.5 in mice: one NSC lineage produces cells only for the cortex and another provides neurons to the olfactory bulb. The majority of neurosphere-forming NSCs in the adult brain are relatively dormant and generate very few cells, if any, in the olfactory bulb or cortex, and this NSC population could serve as a reservoir that is occasionally reactivated later in life. |
| Databáze: | OpenAIRE |
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