| Autor: |
Rene Geissler, Nancy Funk, Jan van Lunzen, Jan Chemnitz, Martina Behrens, Sven-Erik Behrens, Ilona Hauber, Heidi Baum, Ivonne Renner, Helga Hofmann-Sieber, Annekatrin Richter, Jens Boch, Joachim Hauber |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Virology. 486 |
| ISSN: |
1096-0341 |
| Popis: |
The major obstacle to cure infections with human immunodeficiency virus (HIV-1) is integrated proviral genomes, which are not eliminated by antiretroviral therapies (ART). Treatment approaches with latency-reversing agents (LRAs) aim at inducing provirus expression to tag latently-infected cells for clearance through viral cytopathic effects or cytotoxic T cell (CTL) responses. However, the currently tested LRAs reveal evident drawbacks as gene expression is globally induced and viral outgrowth is insecure. Here, we present transcription activator-like effector (TALE) proteins as potent tools to activate HIV-1 specifically. The large variety of circulating HIV-1 strains and, accordingly, integrated proviruses was addressed by the programmable DNA-specificity of TALEs. Using customized engineered TALEs, a substantial transcription activation and viral outgrowth was achieved with cells obtained from different HIV-1 patients. Our data suggest that TALEs may be useful tools in future strategies aimed at removing HIV-1 reservoirs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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