A regulatory toolbox of MiniPromoters to drive selective expression in the brain
| Autor: | Bibiana K. Y. Wong, Randy Glenn, Cletus D'Souza, Vik Chopra, Magdalena I. Swanson, Meifen Lu, Flora Liu, Douglas J. Swanson, Marko Milisavljevic, Diana L. Palmquist, Taryn G. Hearty, Jenna L. Turner, Jonathan S. Lim, Steven J.M. Jones, Debra L. Fulton, Erin K. Flynn, Wyeth W. Wasserman, Dan Goldowitz, Betty Palma, Sonia F. Black, David J. Arenillas, Steven Jiang, Jing M. Chen, Charles N. de Leeuw, Russell J. Bonaguro, Stéphanie Laprise, Kathleen G. Banks, Athena R. Ypsilanti, Jacek Mis, Mahsa Amirabbasi, Ivana Komljenovic, Katie O’Connor, Robert A. Holt, Nancy Y. Liao, Ying Chen, Russell F. Watkins, Stuart Lithwick, Shannan J. Ho Sui, Elodie Portales-Casamar, Richard Varhol, Bonny Tam, Jun Liu, Li Liu, Lisa Dreolini, Mauro Castellarin, Andrea J. McLeod, Amy Ticoll, Nazar Babyak, Kristi Hatakka, Elizabeth M. Simpson, Jason C. Y. Cheng, Gary M. Wilson, Siaw H. Wong, Erich Brauer, Jean-François Schmouth, Behzad Imanian, Melissa K. McConechy, Johar Ali, Shadi Khorasan-zadeh, Jenny Vermeulen, T. Roderick Docking, George S. Yang, Tony Wong, Tara Candido |
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| Rok vydání: | 2010 |
| Předmět: |
Genetically modified mouse
Cellular differentiation Gene Expression Mice Transgenic Computational biology Biology Regulatory Sequences Nucleic Acid Mice Genes Reporter Gene expression Databases Genetic Animals Humans Gene Knock-In Techniques Promoter Regions Genetic Gene Embryonic Stem Cells Genetics Neurons Reporter gene Multidisciplinary Gene Expression Profiling Brain Computational Biology Cell Differentiation Genomics Biological Sciences Embryonic stem cell Gene expression profiling Regulatory sequence |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 107(38) |
| ISSN: | 1091-6490 |
| Popis: | The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression patterns of interest are subjected to bioinformatic analysis to delineate candidate regulatory regions, which are then incorporated into a panel of compact human MiniPromoters to drive expression to brain regions and cell types of interest. Using single-copy, homologous-recombination “knockins” in embryonic stem cells, each MiniPromoter reporter is integrated immediately 5′ of the Hprt locus in the mouse genome. MiniPromoter expression profiles are characterized in differentiation assays of the transgenic cells or in mouse brains following transgenic mouse production. Histological examination of adult brains, eyes, and spinal cords for reporter gene activity is coupled to costaining with cell-type–specific markers to define expression. The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies. |
| Databáze: | OpenAIRE |
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