Th2-Biased Immune Responses Are Important in a Murine Model of Chronic Hypersensitivity Pneumonitis
| Autor: | Keiko Mitaka, Naohiko Inase, Yasunari Miyazaki, Masashi Furuie, Shuji Miyake, Yasuyuki Yoshizawa, Makito Yasui |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Pulmonary Fibrosis Immunology Mice Inbred Strains Mice Th2 Cells Species Specificity Fibrosis Immunopathology Pulmonary fibrosis medicine Animals Humans Immunology and Allergy Lung Mice Inbred BALB C medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Respiratory disease Interleukin General Medicine medicine.disease Immunohistochemistry Mice Inbred C57BL Disease Models Animal Bronchoalveolar lavage medicine.anatomical_structure Chronic Disease Cytokines Female business Bronchoalveolar Lavage Fluid Hypersensitivity pneumonitis Alveolitis Extrinsic Allergic |
| Zdroj: | International Archives of Allergy and Immunology. 154:264-274 |
| ISSN: | 1423-0097 1018-2438 |
| Popis: | Background: Chronic hypersensitivity pneumonitis (HP) can lead to irreversible pulmonary fibrosis. A good animal model is essential to elucidate the mechanisms of this disease. We previously reported that a Th2 predominance may play an important role in the fibrogenesis in chronic HP patients. A study was undertaken to evaluate whether Th2-biased immune responses were crucial during the processes of lung fibrosis in a murine model of chronic HP. Methods: Instillation of pigeon dropping extracts (PDE) was conducted 3 days a week for 6 or 12 weeks in C57BL/6, BALB/c and A/J mice to establish models of chronic HP. We evaluated the histopathological features, immunohistochemistry, collagen content, bronchoalveolar lavage fluid (BALF) profiles and Th1/Th2 cytokines in BALF or lung tissue with RT-PCR and ELISA. Results: Thickening of the alveolar walls and structural alterations were observed only in the A/J mice after 12 weeks of exposure to PDE. The fibrosis scores were significantly increased in 12-week A/J mice compared to those in the other strains. Immunohistochemistry evaluation showed that PDE was engulfed by alveolar macrophages that were incorporated into the alveolar septa of 12-week A/J mice. Interleukin (IL)-4 mRNA increased significantly in 6- and 12-week A/J mice. IL-13 mRNA showed a significant increase in 12-week A/J mice compared with 6-week A/J mice. TGF-β1 mRNA at 12 weeks was significantly increased in A/J mice compared with the other groups. Conclusion: Th2-biased genetic backgrounds may play an important role in fibrosing processes in the present chronic HP model. |
| Databáze: | OpenAIRE |
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