BB rat Gimap gene expression in sorted lymphoid T and B cells
| Autor: | Daniel H. Moralejo, William R. A. Osborne, Richard A. Jensen, Jessica M. Fuller, Anne E. Kwitek, Brian Van Yserloo, Ruth A. Ettinger, Åke Lernmark, Elizabeth A. Rutledge |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
T-Lymphocytes
T cell Quantitative Trait Loci Autoimmunity CD8-Positive T-Lymphocytes Endocrinology and Diabetes Biology Quantitative trait locus medicine.disease_cause Polymerase Chain Reaction Article General Biochemistry Genetics and Molecular Biology Frameshift mutation 03 medical and health sciences 0302 clinical medicine GTP-Binding Proteins immune system diseases Lymphopenia Gene expression medicine Animals Gene family Rats Inbred BB General Pharmacology Toxicology and Pharmaceutics 030304 developmental biology Genetics Regulation of gene expression B-Lymphocytes 0303 health sciences Thymocytes General Medicine Rats Thymocyte Diabetes Mellitus Type 1 Type 1 diabetes medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis Gimap Lymph Nodes Spleen |
| Zdroj: | Life Sciences; 89(19-20), pp 748-754 (2011) |
| ISSN: | 0024-3205 1879-0631 |
| Popis: | Aims: The Gimap gene family has been shown to be integral to T cell survival and development. A frameshift mutation in Gimap5, one of seven members of the Gimap family, results in lymphopenia and is a prerequisite for spontaneous type 1 diabetes (T1D) in the BioBreeding (BB) rat. While not contributing to lymphopenia, the Gimap family members proximal to Gimap5, encompassed within the Iddm39 quantitative trait locus (QTL), have been implicated in T1D. We hypothesized that expression of the Gimap family members within the Iddm39 QTL, during thymocyte development as well as in peripheral T and B cells contribute to T1D. Main methods: Cell sorted subpopulations were analyzed by quantitative real time (qRT) PCR. Key findings: Gimap4 expression was reduced in DR.(lyp/lyp) rat double negative, double positive and CD8 single positive (SP) thymocytes while expression of Gimap8, Gimap6, and Gimap7 was reduced only in CD8 SP thymocytes. Interestingly, expression of the entire Gimap gene family was reduced in DR.(lyp/lyp) rat peripheral T cells compared to non-lymphopenic, non-diabetic DR.(+/+) rats. With the exception of Gimap6. the Gimap family genes were not expressed in B cells from spleen and mesenteric lymph node (MLN). Expression of Gimap9 was only detected in hematopoietic cells of non B cell lineage such as macrophage, dendritic or NK cells. Significance: These results suggest that lack of the Gimap5 protein in the DR.(lyp/lyp) congenic rat was associated with impaired expression of the entire family of Gimap genes and may regulate T cell homeostasis in the peripheral lymphoid organs. (C) 2011 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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