Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
| Autor: | Karen E. DeMatteo, Miguel A. Rinas, Franco F. Roldán Gallardo, Carina F. Argüelles |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Jaguar Rodent Guinea Pigs lcsh:Medicine Cavia Rodentia 010603 evolutionary biology 01 natural sciences General Biochemistry Genetics and Molecular Biology SALIVA CUNICULUS PACA HETEROLOGOUS AMPLIFICATION purl.org/becyt/ford/1 [https] 03 medical and health sciences Cuniculus paca Cuniculidae biology.animal Scat MICROSATELLITE PRIMERS Animals Panthera Paca lcsh:Science (General) purl.org/becyt/ford/1.6 [https] Saliva lcsh:QH301-705.5 Genetic diversity Heterologous amplification biology Ecology lcsh:R General Medicine Panthera onca biology.organism_classification Wildlife trade Research Note 030104 developmental biology Microsatellite primers lcsh:Biology (General) SCAT Microsatellite Puma lcsh:Q1-390 Microsatellite Repeats |
| Zdroj: | BMC Research Notes CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET BMC Research Notes, Vol 13, Iss 1, Pp 1-7 (2020) |
| ISSN: | 1756-0500 |
| Popis: | Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade. Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina Fil: Dematteo, Karen. Wildcare Institute at the Saint Louis Zoo; Estados Unidos. Washington University in St. Louis; Estados Unidos Fil: Rinas, Miguel Angel. Gobierno de la Provincia de Misiones. Ministerio de Ecología y Recursos Naturales Renovables; Argentina Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina |
| Databáze: | OpenAIRE |
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