A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast
| Autor: | Herbert Auer, Léon Dirick, Kim Nasmyth, Thomas Moll |
|---|---|
| Přispěvatelé: | Research Institute of Molecular Pathology (IMP) |
| Rok vydání: | 1992 |
| Předmět: |
Transcription
Genetic [SDV]Life Sciences [q-bio] MESH: Cell Cycle MESH: DNA Replication DNA-Directed DNA Polymerase MESH: Base Sequence chemistry.chemical_compound MESH: Saccharomyces cerevisiae Proteins Transcription (biology) MESH: DNA-Directed DNA Polymerase DNA Fungal Deoxyribonucleases Type II Site-Specific Promoter Regions Genetic MESH: Bacterial Proteins MESH: Deoxyribonucleases Type II Site-Specific 0303 health sciences Multidisciplinary 030302 biochemistry & molecular biology Cell Cycle MESH: Transcription Factors Cell cycle MESH: Saccharomyces cerevisiae Cell biology MESH: beta-Galactosidase MESH: Fungal Proteins MESH: Thymidylate Synthase DNA Replication Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Molecular Sequence Data Biology MESH: G1 Phase Fungal Proteins 03 medical and health sciences Bacterial Proteins MESH: Promoter Regions Genetic [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Gene Transcription factor 030304 developmental biology MESH: Molecular Sequence Data Binding Sites Base Sequence MESH: Transcription Genetic DNA replication G1 Phase Promoter Thymidylate Synthase biology.organism_classification beta-Galactosidase Molecular biology MESH: DNA Fungal chemistry MESH: Binding Sites DNA Transcription Factors |
| Zdroj: | Nature Nature, Nature Publishing Group, 1992, 357 (6378), pp.508-513. ⟨10.1038/357508a0⟩ |
| ISSN: | 0028-0836 1476-4679 |
| DOI: | 10.1038/357508a0⟩ |
| Popis: | International audience; Most genes involved in DNA replication in the yeast Saccharomyces cerevisiae are transcribed transiently during late G1 as cells become committed to a new cell cycle at Start. Their promoters all contain one or more versions of an 8-base-pair motif (ACGCGTNA) containing an MluI restriction enzyme site and called the MluI cell-cycle box (MCB). MCBs are both necessary and sufficient for the late G1-specific transcription of the TMP1 thymidylate synthase and POL1 DNA polymerase genes. A different late G1-specific 8-base-pair transcription element called the SCB (CACGAAAA; ref. 5) is bound by a factor containing the Swi4 and Swi6 proteins. We describe here the formation in vitro of complexes on TMP1 MCBs that contain the Swi6 protein and, we suggest, a protein of relative molecular mass 120,000 (p120) that is distinct from Swi4. Transcription due to SCBs and MCBs occurs in the absence of Swi6 but it is no longer correctly regulated in the cell cycle. We suggest that Swi6 is an essential regulatory subunit of two different Start-dependent transcription factors. One factor (SBF) contains Swi4 and binds to SCBs, whereas the other (MBF) contains the protein p120 and binds MCBs. |
| Databáze: | OpenAIRE |
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