Autoantibody profiling of Chinese patients with autoimmune hepatitis using immunoproteomic analysis
| Autor: | Ming-Yuan Li, Jie Wang, Mei-Ru Hu, Yan Zhao, Yan Xue, Yuan-Fang Ma, Beifen Shen, Ning Guo, Bing-Yv Liu, Hongxia Wang, Qing Xia, Song-Cheng Yang, Hui-Ping Yan, Feng Lu, Lu Qian, Xia Feng, Bo-An Li, Xue-Min Zhang, Ping Li |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Adolescent Proteome Molecular Sequence Data Autoimmune hepatitis Biochemistry Autoantigens Serology law.invention Primary biliary cirrhosis Antigen Asian People immune system diseases law Cell Line Tumor medicine Animals Humans Amino Acid Sequence Aged Autoantibodies Aged 80 and over business.industry Autoantibody General Chemistry Middle Aged medicine.disease digestive system diseases Hepatitis Autoimmune Fumarase Immunology Recombinant DNA Female business Viral hepatitis |
| Zdroj: | Journal of proteome research. 7(5) |
| ISSN: | 1535-3893 |
| Popis: | In the present study, immunoproteomic analysis was utilized to systemically characterize global autoantibody profiles in autoimmune hepatitis (AIH). Sera from 21 patients with AIH and 15 healthy controls were analyzed for the antibody reactivity against the protein antigens of HepG2, a human hepatoma cell line. The lysates of HepG2 cells were separated by two-dimensional electrophoresis and then immunoblotted with each serum sample. Matrix-assisted laser desorption/ionization mass spectrometry or/and nanoelectrospray ionization MS/MS were then used to identify antigens, among which a bifunctional enzyme in mitochondrial, fumarate hydratase (FH), was further analyzed by ELISA using recombinant FH as a coating antigen. A total of 18 immunoreactive spots were identified as 13 proteins, 8 of which have not been reported in AIH. Immune reactivity to FH was detected in 66.67% of patients with AIH, 19.35% of patients with primary biliary cirrhosis (PBC), 12.31% of patients with chronic hepatitis B (CHB), 6.35% of patients with chronic hepatitis C (CHC), 11.32% of patients with systemic lupus erythematosus (SLE), and 3.57% of normal individuals. The differences of prevalence between AIH patients and healthy controls as well as other diseases were of statistical significance (P |
| Databáze: | OpenAIRE |
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