Effect of castration on pelvic neurons in the male pig
| Autor: | Agnieszka Dudek, Z. Pidsudko, Waldemar Sienkiewicz, Natalia Kasica-Jarosz, Jerzy Kaleczyc, M. Klimczuk |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Histology medicine.drug_class Swine Adrenergic Calcitonin gene-related peptide Biology Neuroprotection Pelvis 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Animals Galanin Cholinergic neuron Molecular Biology Neurons 030102 biochemistry & molecular biology Cell Biology Androgen Medical Laboratory Technology 030104 developmental biology Castration Endocrinology nervous system chemistry Immunohistochemistry RNA Ganglia Orchiectomy |
| Zdroj: | Histochemistry and cell biology. 153(3) |
| ISSN: | 1432-119X |
| Popis: | The present study investigated the influence of castration performed at neonatal age on neuronal elements in the anterior pelvic ganglion of the male pig with immunohistochemistry and quantitative real-time PCR (qPCR). The ganglia were examined 3 and 6 months after surgery. In 3-month-old castrated pigs (3MCP) 74% of adrenergic and 31% of cholinergic neurons stained for caspase-3 (CASP-3), and much greater numbers of perikarya than in the control animals expressed CGRP, galanin (GAL) and VIP (peptides known to have neuroprotective properties). In 6-months-old castrated pigs (6MCP), an excessive loss (90%) of neurons and intraganglionic nerve fibres was found. The survived adrenergic and cholinergic neurons also expressed CASP-3, CGRP, GAL or VIP. The qPCR results corresponded with immunofluorescence findings. In 3MCP, genes for CASP-3 and CGRP were up-regulated, while the expression of those for DβH, VAChT, GAL, VIP and SP displayed statistically insignificant variations. In 6MCP, distinctly up-regulated were genes for CGRP, GAL, VIP, SP, DβH and VAChT, while the expression of casp3 gene was down-regulated. The study revealed for the first time the excessive loss of pelvic neurons following castration, and a realistic assumption is proposed, that the neurons died due to apoptosis triggered by androgen deprivation. |
| Databáze: | OpenAIRE |
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