A zeptoliter volume meter for analysis of single protein molecules
| Autor: | Stefan Wennmalm, Horst Vogel, Tor Sandén, Christian Santschi, Olivier J. F. Martin, Cédric Deluz, Romain Wyss, Ghérici Hassaine |
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| Rok vydání: | 2011 |
| Předmět: |
Nanostructure
Aperture Protein Conformation Diffusion Analytical chemistry Nanoparticle Ultrafiltration Bioengineering Fluorescence correlation spectroscopy 02 engineering and technology 03 medical and health sciences Nanotechnology General Materials Science Particle Size 030304 developmental biology chemistry.chemical_classification 0303 health sciences Aqueous solution Chemistry Mechanical Engineering Biomolecule Proteins General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence Spectrometry Fluorescence Nanoparticles 0210 nano-technology Porosity |
| Zdroj: | Nano Letters |
| ISSN: | 1530-6992 |
| Popis: | A central goal in bioanalytics is to determine the concentration of and interactions between biomolecules. Nanotechnology allows performing such analyses in a highly parallel, low-cost, and miniaturized fashion. Here we report on label-free volume, concentration, and mobility analysis of single protein molecules and nanoparticles during their diffusion through a subattoliter detection volume, confined by a 100 nm aperture in a thin gold film. A high concentration of small fluorescent molecules renders the aqueous solution in the aperture brightly fluorescent. Nonfluorescent analytes diffusing into the aperture displace the fluorescent molecules in the solution, leading to a decrease of the detected fluorescence signal, while analytes diffusing out of the aperture return the fluorescence level. The resulting fluorescence fluctuations provide direct information on the volume, concentration, and mobility of the nonfluorescent analytes through fluctuation analysis in both time and amplitude. |
| Databáze: | OpenAIRE |
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