Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production
| Autor: | Guido Heine, T. Zuberbier, Margitta Worm, Milena Milovanovic |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Allergy
medicine.medical_treatment Immunology Receptors Antigen B-Cell medicine.disease_cause Immunoglobulin E Allergen Antigen immune system diseases otorhinolaryngologic diseases medicine Humans Immunology and Allergy B-Lymphocytes biology Allergen extract Allergens medicine.disease Interleukin-10 Interleukin 10 Cytokine Toll-Like Receptor 9 biology.protein Antibody Immunologic Memory |
| Zdroj: | Clinical & Experimental Allergy. 39:671-678 |
| ISSN: | 1365-2222 0954-7894 |
| Popis: | Summary Background Elevated specific IgE antibody levels are common in atopic individuals, caused by T-helper type 2-dominated B cell activation. The induction of antigen-specific IL-10 secreting T cells is discussed as an important mechanism during specific immunotherapy. By contrast the presence and function of B cell-derived IL-10 is not well defined yet. Objective We investigated whether type-I allergen extracts induce IL-10 expression in human B cells and analysed its functional role on IgE production. Methods Human peripheral B cells were stimulated with grass pollen, house dust mite (HDM) (Dermatophagoides pteronyssimus; Der p) and dog allergen extract. Expression of IL-10 by activated human B cells was determined by flow cytometric analysis and ELISA. Functional analysis considering immunoglobulin production was assayed by ELISA. Results The allergen extracts studied induced IL-10 expression in B cells. However, the ability to induce IL-10 differed between the allergen extracts. The most potent allergen extract was dog (169±28 pg/mL), followed by grass pollen (141±10 pg/mL) and HDM allergen (125±11 pg/mL). Upon allergen extract stimulation only CD27 expressing memory B cells produced IL-10 and co-expressed the very early activation antigen CD69. The addition of allergen extracts to B cells activated by anti-CD40 and IL-4 selectively inhibited IgE which was dependent on allergen extract-induced IL-10. By contrast the other immunoglobulin subclasses like IgA, IgG or IgM were not altered upon allergen extract challenge. Conclusion Our data indicate that allergen-activated memory B cells can modulate IgE production through secretion of IL-10. |
| Databáze: | OpenAIRE |
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