Development and Multicenter Evaluation of the N Latex CDT Direct Immunonephelometric Assay for Serum Carbohydrate-Deficient Transferrin
| Autor: | Heinz Jürgen Roth, Catherine Born, Anders Helander, Joris R. Delanghe, Wolfgang K. Gentzer, François Schellenberg, J. Maurits Pekelharing, Jos P.M. Wielders, Eray Yagmur, Harald Althaus |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Glycosylation Adolescent Clinical Biochemistry Carbohydrate deficient transferrin Sensitivity and Specificity High-performance liquid chromatography Mice Congenital Disorders of Glycosylation Nephelometry and Turbidimetry Reference Values medicine Animals Humans False Positive Reactions Child Immunoassay chemistry.chemical_classification Mice Inbred BALB C Autoanalysis medicine.diagnostic_test biology Biochemistry (medical) Transferrin Genetic variants Antibodies Monoclonal Genetic Variation Serum samples Molecular biology Alcoholism chemistry Homogeneous Immunology biology.protein Female Antibody Biomarkers |
| Zdroj: | Clinical Chemistry. 53:1115-1121 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/clinchem.2006.084459 |
| Popis: | Background: Carbohydrate-deficient transferrin (CDT) is a promising biomarker of alcohol abuse. We describe the development and multicenter evaluation of N Latex CDT (Dade Behring), an automated, particle-enhanced, homogeneous immunonephelometric assay for directly determining CDT.Methods: N Latex CDT uses a monoclonal antibody that recognizes the structure of transferrin glycoforms lacking 1 or 2 complete N-glycans [i.e., disialo-, monosialo-, and asialotransferrins (CDT glycoforms)] in combination with a simultaneous assay for total transferrin. The Dade Behring BN II™ and BN ProSpec® systems automatically calculate the CDT value as a percentage of total transferrin (%CDT). No preanalytical sample treatment is used.Results: Total imprecision values for serum pools containing 1.8%–8.7% CDT were 3.4%–10.4% (mean, 6.8%). The mean (SD) %CDT for 561 serum samples from healthy control individuals was 1.76% (0.27%; range, 1.01%–2.85%). No marked sex or age differences were noted. The 97.5th percentile was at 2.35%. Transferrin genetic variants did not interfere with measurements. High transferrin concentrations did not falsely increase %CDT values, but increased %CDT values were noted for some samples with transferrin concentrations Conclusion: N Latex CDT is the first direct immunoassay for quantifying %CDT in serum. The specificity of N Latex CDT for identifying alcohol abuse may be higher than for immunoassays that use column separation, because transferrin genetic variants do not interfere with measurements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|