Transcriptome analysis of CD34+ cells from myelodysplastic syndrome patients
| Autor: | Qi Zhong, Rui Chen, Zhi Liu, Mengdong Yao, Jing Huang, Qing Zhang, Shanyao Zhou, Liling Zheng, Huijuan Shen, Yanling She, Cheng Li, Ruiming Ou, Yangmin Zhu, Shuang Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research CD34 Antigens CD34 Refractory anemia with ringed sideroblasts Biology Recombination-activating gene Transcriptome 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases medicine Humans Gene Expression Profiling Myeloid leukemia Hematology CD79B medicine.disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Myelodysplastic Syndromes Cancer research Primary immunodeficiency Stem cell |
| Zdroj: | Leukemia research. 62 |
| ISSN: | 1873-5835 |
| Popis: | The myelodysplastic syndrome (MDS) represents a heterogeneous group of clonal hematologic stem cell disorders with the characteristic of ineffective hematopoiesis leading to low blood counts, and a risk of progression to acute myeloid leukemia (AML). To understand specific molecular characteristics of different MDS subtypes with del(5q), we analyzed the gene expression profiles of CD34+ cells from MDS patients of different databases and its enriched pathways. 44 genes, such as MME and RAG1, and eight related pathways were identified to be commonly changed, indicating their conserved roles in MDS development. Additionally, U43604 was identified to be specifically changed in three subtypes with del(5q), including refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS) and refractory anemia with excess blasts (RAEB). C10orf10 and CD79B were specifically changed in RA patients with del(5q), while POU2AF1 were in RARS patients with del(5q). We also analyzed specific pathways of MDS subtypes, such as "Glycosaminoglycan biosynthesis-chondroitin sulfate" which was specific identified in RARS patients. Importantly, those findings can be validated well using another MDS database. Taken together, our analysis identified specific genes and pathways associated with different MDS subtypes with del(5q). |
| Databáze: | OpenAIRE |
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