Performance of VITEK 2, E-test, Kirby-Bauer disk diffusion, and modified Kirby-Bauer disk diffusion compared to reference broth microdilution for testing tigecycline susceptibility of carbapenem-resistant K. pneumoniae and A. baumannii in a multicenter study in China
| Autor: | Ying Liu, Jingyong Sun, Jin Tang, Fupin Hu, Yuxing Ni, Hong Zhang, Dandan Yin, Feng Chen, Yan Guo, Wen-Juan Wu, Min Li, Hu Zhao |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Acinetobacter baumannii China Klebsiella pneumoniae 030106 microbiology Tigecycline Microbial Sensitivity Tests Microbiology 03 medical and health sciences 0302 clinical medicine Disk Diffusion Antimicrobial Tests Drug Resistance Bacterial Medicine Humans 030212 general & internal medicine Agar diffusion test biology business.industry Broth microdilution K pneumoniae General Medicine biology.organism_classification Antimicrobial Anti-Bacterial Agents Klebsiella Infections Infectious Diseases Multicenter study Carbapenems business medicine.drug Acinetobacter Infections |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 40(6) |
| ISSN: | 1435-4373 |
| Popis: | Tigecycline is an alternative antibiotic for managing carbapenem-resistant Gram-negative bacterial infections. However, disk diffusion and automated testing often show false-intermediate or false-resistant results in tigecycline susceptibility, misleading clinical antimicrobial therapy. Broth microdilution (BMD) is the reference method for testing tigecycline susceptibility, but it is labor intensive and time consuming to perform in clinical laboratories. Therefore, a simple and accurate method is urgently needed. We evaluated the performance of VITEK 2, E-test, Kirby-Bauer disk diffusion (KB), and modified KB disk diffusion (mKB) versus BMD in testing tigecycline susceptibility of 372 strains of carbapenem-resistant Klebsiella pneumoniae (CRKP) and 346 strains of carbapenem-resistant Acinetobacter baumannii (CRAB). BMD confirmed that 96.8% of CRKP and 91% of CRAB strains were susceptible to tigecycline. E-test, VITEK 2, KB, and mKB yielded categorical agreement of 96.7/59.3%, 69.9/54.3%, 78.5/87.3%, and 96.5%/91% for CRKP/CRAB, respectively. No very major error was found for either CRKP or CRAB by any method. No major error was found for CRKP or CRAB by the mKB method. The mKB method enhanced by R-buffer is simple, accurate, and inexpensive for clinical laboratories to test the susceptibility of CRKP and CRAB isolates to tigecycline. |
| Databáze: | OpenAIRE |
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