Dual growth factor delivery using PLGA nanoparticles in silk fibroin/PEGDMA hydrogels for articular cartilage tissue engineering
| Autor: | Milad Fathi-Achachelouei, Nihal Engin Vrana, Aysen Tezcaner, Erhan Bat, Dilek Keskin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular Materials science Cell Survival medicine.medical_treatment Basic fibroblast growth factor Biomedical Engineering Fibroin Biocompatible Materials cartilage tissue engineering 02 engineering and technology Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols Prosthesis Implantation Transforming Growth Factor beta1 Biomaterials 03 medical and health sciences chemistry.chemical_compound Nanocapsules Tissue engineering Dental pulp stem cells TGF-β1 medicine Humans Collagen Type II Glycosaminoglycans 030304 developmental biology 0303 health sciences Tissue Engineering Tissue Scaffolds Growth factor technology industry and agriculture Cell Differentiation 021001 nanoscience & nanotechnology Chondrogenesis Drug Liberation PLGA chemistry bFGF Self-healing hydrogels Mechanical Tests Biophysics Methacrylates nanoparticles hydrogel Fibroins 0210 nano-technology Antimicrobial Cationic Peptides |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials |
| Popis: | Degeneration of articular cartilage due to damages, diseases, or age-related factors can significantly decrease the mobility of the patients. Various tissue engineering approaches which take advantage of stem cells and growth factors in a threedimensional constructs have been used for reconstructing articular tissue. Proliferative impact of basic fibroblast growth factor (bFGF) and chondrogenic differentiation effect of transforming growth factor-beta 1 (TGF-β1) over mesenchymal stem cells have previously been verified. In this study, silk fibroin (SF) and of poly(ethylene glycol) dimethacrylate (PEGDMA) were used to provide a versatile platform for preparing hydrogels with tunable mechanical, swelling and degradation properties through physical and chemical crosslinking as a microenvironment for chondrogenic differentiation in the presence of bFGF and TGF-β1 releasing nanoparticles (NPs) for the first time. Scaffolds with compressive moduli ranging from 95.70 ± 17.82 to 338.05 ± 38.24 kPa were obtained by changing both concentration PEGDMA and volume ratio of PEGDMA with 8% SF. Highest cell viability was observed in PEGDMA 10%- SF 8% (1:1) [PEG10-SF8(1:1)] hydrogel group. Release of bFGF and TGF-β1 within PEG10-SF8(1:1) hydrogels resulted in higher DNA and glycosaminoglycans amounts indicating synergistic effect of dual release over proliferation and chondrogenic differentiation of dental pulp stem cells in hydrogels, respectively. Our results suggested that simultaneous delivery of bFGF and TGF-β1 through utilization of PLGA NPs within PEG10-SF8(1:1) hydrogel provided a novel and versatile means for articular cartilage regeneration as they allow for dosage- and site-specific multiple growth factor delivery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|