Mining the unmapped reads in bovine RNA-Seq data reveals the prevalence of bovine herpes virus-6 in European dairy cows and the associated changes in their phenotype and leucocyte transcriptome
| Autor: | Laura Buggiotti, Zhangrui Cheng, Alessandra Crisà, Dirk Werling, HEDI HAMMAMI, Tine Rousing, Federica Signorelli, Mark Crowe, Leslie Foldager |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endometritis 040301 veterinary sciences lcsh:QR1-502 RNA-Seq Biology mastitis Article lcsh:Microbiology Microbiology 0403 veterinary science Transcriptome 03 medical and health sciences Immune system Gammaherpesvirinae Interferon Virology medicine Leukocytes Prevalence Animals dairy cows Gene bovine herpes virus-6 BoHV-4 RNA 04 agricultural and veterinary sciences interferon medicine.disease Phenotype Mastitis 030104 developmental biology Infectious Diseases ISGylation Cattle Female leukocyte medicine.drug |
| Zdroj: | Buggiotti, L, Cheng, Z, Wathes, D C, GplusE Consortium & Ingvartsen, K L 2020, ' Mining the unmapped reads in bovine RNA-Seq data reveals the prevalence of bovine herpes virus-6 in European dairy cows and the associated changes in their phenotype and leucocyte transcriptome ', Viruses, vol. 12, no. 12, 1451 . https://doi.org/10.3390/v12121451 Viruses Volume 12 Issue 12 Viruses, Vol 12, Iss 1451, p 1451 (2020) |
| ISSN: | 1999-4915 |
| Popis: | Microbial RNA is detectable in host samples by aligning unmapped reads from RNA\ud sequencing against taxon reference sequences, generating a score proportional to the microbial load.\ud An RNA-Seq data analysis showed that 83.5% of leukocyte samples from six dairy herds in different\ud EU countries contained bovine herpes virus-6 (BoHV-6). Phenotypic data on milk production,\ud metabolic function, and disease collected during their first 50 days in milk (DIM) were compared\ud between cows with low (1–200 and n = 114) or high (201–1175 and n = 24) BoHV-6 scores. There were\ud no differences in milk production parameters, but high score cows had numerically fewer\ud incidences of clinical mastitis (4.2% vs. 12.2%) and uterine disease (54.5% vs. 62.7%). Their metabolic\ud status was worse, based on measurements of IGF-1 and various metabolites in blood and milk. A\ud comparison of the global leukocyte transcriptome between high and low BoHV-6 score cows at\ud around 14 DIM yielded 485 differentially expressed genes (DEGs). The top pathway from Gene\ud Ontology (GO) enrichment analysis was the immune system process. Down-regulated genes in the\ud high BoHV-6 cows included those encoding proteins involved in viral detection (DDX6 and\ud DDX58), interferon response, and E3 ubiquitin ligase activity. This suggested that BoHV-6 may\ud largely evade viral detection and that it does not cause clinical disease in dairy cows. |
| Databáze: | OpenAIRE |
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