Microanatomical changes and biomolecular expression at the PDL ‐entheses during experimental tooth movement
| Autor: | Arvin Pal, Andrew H. Jheon, Bo Meng, Misun Kang, Sunita P. Ho, Ling Chen, Rui He, Lynn Yang |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Molar Bone sialoprotein Tooth Movement Techniques Periodontal Ligament CD146 Antigen Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system medicine Animals Integrin-Binding Sialoprotein Regeneration Periodontal fiber Dental Cementum biology Chemistry X-Ray Microtomography 030206 dentistry Enthesis Resorption Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Osmium tetroxide Sp7 Transcription Factor Ultrastructure biology.protein Ligament Periodontics Tooth Mobility Ubiquitin Thiolesterase |
| Zdroj: | J Periodontal Res |
| ISSN: | 1600-0765 0022-3484 |
| Popis: | The novel aspect of this study is to contextualize the co-localization of biomolecular expression in widened and narrowed periodontal ligament (PDL) space within a mechanically activated periodontal complex. The PDL is unique as it is the only ligament with both innervation and vascularization. Maxillary molars in 6-week old male C57BL/6 mice (N=5) were experimentally translated for two weeks using an elastic spacer. Contralateral teeth were used as controls. Mechanical testing of the periodontal complex of a mouse in situ and imaging using an X-ray micro-computed tomography (micro-XCT) illustrated deformations within blood vessels (BV) of the PDL under load. PDL-bone and PDL-cementum entheses at the widened and narrowed PDL-spaces following experimental tooth movement (ETM) illustrated osterix (OSX), bone sialoprotein (BSP), cluster of differentiation 146 (CD146), and protein gene product 9.5 (PGP9.5), indicating active remodeling at these sites. PGP9.5 positive nerve bundles (NBs) were co-localized with multinucleated cells (MCs), Howship’s resorption lacunae, and CD146 positive BVs. Association between nerves and MC was complemented by visualizing the proximity of osmium tetroxide stained NBs with the ultrastructure of MCs by performing scanning transmission electron microscopy. Spatial association of NB with BV, and NB with MC, provided insights into the plausible co-activation of NBs to initiate osteoclastic activity. Resorption of mineral occurs as an attempt to restore PDL-space of the load bearing complex, specifically at the PDL-entheses. Mapping of anatomy-specific structural elements and their association with regenerative molecules by correlating light and electron micrographs can provide insights into the use of these extracellular matrix molecules as plausible targets for pharmacological interventions related to tooth movement. Within the realm of tissue regeneration, modulation of load can reverse naturally occurring mineral formation to experimentally-induced resorption, and naturally occurring mineral resorption to experimentally-induced formation at the enthesial sites in order to permit tooth translation. |
| Databáze: | OpenAIRE |
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