STAT3 and importins are novel mediators of early molecular and cellular responses in experimental duodenal ulceration
| Autor: | Khushin N. Patel, Sandor Szabo, Xiaoming Deng, Zsuzsanna Sandor, Andrzej S. Tarnawski, Tetyana Khomenko, Amrita Ahluwalia |
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| Rok vydání: | 2013 |
| Předmět: |
STAT3 Transcription Factor
alpha Karyopherins Time Factors Physiology Duodenum Cysteamine Active Transport Cell Nucleus Apoptosis Rats Sprague-Dawley chemistry.chemical_compound Mice Animals SOCS3 Epirizole Phosphorylation STAT3 Transcription factor Early Growth Response Protein 1 Mice Knockout biology Gastroenterology Tyrosine phosphorylation beta Karyopherins Rats Disease Models Animal chemistry Gene Expression Regulation Oligodeoxyribonucleotides Duodenal Ulcer Knockout mouse STAT protein Cancer research biology.protein Tyrosine Female Signal Transduction |
| Zdroj: | Digestive diseases and sciences. 59(2) |
| ISSN: | 1573-2568 |
| Popis: | Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that directly upregulates VEGF, Ref-1, p21, and anti-apoptotic genes such as Bcl-xL. In this study, we hypothesized that STAT3 signaling is activated and provides a critical protective role that is required for enterocyte survival during the early phases of cysteamine-induced duodenal ulcers. We studied the effect of inhibition of STAT3 activity on cysteamine-induced duodenal ulcers in rats and egr-1 knockout mice using STAT3/DNA binding assay, immunohistochemistry, immunoblot, and quantitative reverse transcriptase PCR analyses. We found that G-quartet oligodeoxynucleotides T40214, a specific inhibitor of STAT3/DNA binding, aggravated cysteamine-induced duodenal ulcers in rats 2.8-fold (p |
| Databáze: | OpenAIRE |
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