Anti-allergic property of 4,8-sphingadienine stereoisomers in vivo and in vitro model
| Autor: | Masaki Kuse, Masashi Mizuno, Haruka Shimizu, Ken-ichiro Minato |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Biophysics
Pharmacology Glucosylceramides Inhibitory postsynaptic potential Immunoglobulin E Biochemistry Cell Degranulation Mast cell In vivo Type I allergy Cell Line Tumor Anti-Allergic Agents medicine Animals Edema Humans Mast Cells Molecular Biology Sensitization Mice Inbred BALB C Anti-allergic activity Molecular Structure biology Sphingoid base Plant Extracts Chemistry Passive Cutaneous Anaphylaxis Degranulation Ear Stereoisomerism Biological activity Cell Biology Rats medicine.anatomical_structure Ethanolamines biology.protein Glucosylceramide Female Caco-2 Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 577:32-37 |
| ISSN: | 0006-291X |
| Popis: | 4,8-Sphingadienines (SD), metabolites of glucosylceramides (GlcCer), are sometimes determined as key mediators of the biological activity of dietary GlcCer, and cis/trans geometries of 4,8-SD have been reported to affect its activity. Since regulating excessive activation of mast cells seems an important way to ameliorate allergic diseases, this study was focused on cis/trans stereoisomeric-dependent inhibitory effects of 4,8-SD on mast cell activation. Degranulation of RBL-2H3 was inhibited by treatment of 4-cis-8-trans- and 4-cis-8-cis-SD, and their intradermal administrations ameliorated ear edema in passive cutaneous anaphylaxis reaction, but 4-trans-8-trans- and 4-trans-8-cis-SD did not. Although the activation of mast cells depends on the bound IgE contents, those stereoisomers did not affect IgE contents on RBL-2H3 cells after the sensitization of anti-TNP IgE. These results indicated that 4-cis-8-trans- and 4-cis-8-cis-SD directly inhibit the activation of mast cells. In conclusion, it was assumed that 4,8-SD stereoisomers with cis double bond at C4-position shows anti-allergic activity by inhibiting downstream pathway after activation by the binding of IgE to mast cells. |
| Databáze: | OpenAIRE |
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