Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase
| Autor: | Mithoe, Sharon C., Ludwig, Christina, Pel, Michiel J C, Cucinotta, Mara, Casartelli, Alberto, Mbengue, Malick, Sklenar, Jan, Derbyshire, Paul, Robatzek, Silke, Pieterse, Corné M J, Aebersold, Ruedi, Menke, Frank L H, Dep Biologie, Plant Microbe Interactions, Sub Plant-Microbe Interactions |
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| Přispěvatelé: | Dep Biologie, Plant Microbe Interactions, Sub Plant-Microbe Interactions |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAP Kinase Signaling System Arabidopsis Biology Mitogen-activated protein kinase kinase Biochemistry MAP2K7 03 medical and health sciences Taverne Genetics Plant Immunity ASK1 Phosphorylation Targeted proteomics Molecular Biology MAPK14 Mitogen-Activated Protein Kinase Kinases Innate immunity MAP kinase kinase kinase Arabidopsis Proteins Kinase fungi Pattern recognition receptor Articles Signaling Cell biology 030104 developmental biology bacteria Mitogen-Activated Protein Kinases Reactive Oxygen Species Protein Kinases Flagellin Protein Binding |
| Zdroj: | EMBO Reports, 17, 441. Nature Publishing Group ResearcherID |
| ISSN: | 1469-221X |
| Popis: | Pattern recognition receptors (PRRs) play a key role in plant and animal innate immunity. PRR binding of their cognate ligand triggers a signaling network and activates an immune response. Activation of PRR signaling must be controlled prior to ligand binding to prevent spurious signaling and immune activation. Flagellin perception in Arabidopsis through FLAGELLIN‐SENSITIVE 2 (FLS2) induces the activation of mitogen‐activated protein kinases (MAPKs) and immunity. However, the precise molecular mechanism that connects activated FLS2 to downstream MAPK cascades remains unknown. Here, we report the identification of a differentially phosphorylated MAP kinase kinase kinase that also interacts with FLS2. Using targeted proteomics and functional analysis, we show that MKKK7 negatively regulates flagellin‐triggered signaling and basal immunity and this requires phosphorylation of MKKK7 on specific serine residues. MKKK7 attenuates MPK6 activity and defense gene expression. Moreover, MKKK7 suppresses the reactive oxygen species burst downstream of FLS2, suggesting that MKKK7‐mediated attenuation of FLS2 signaling occurs through direct modulation of the FLS2 complex. |
| Databáze: | OpenAIRE |
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