Multi-site probe for selective turn-on fluorescent detection of Al(III) in aqueous solution: synthesis, cation binding, mode of coordination, logic gate and cell imaging
| Autor: | M. Seenivasaperumal, K.N. Vennila, Gandhi Sivaraman, G. Mahalakshmi, P. Saravana Kumar, Kuppanagounder P. Elango |
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| Rok vydání: | 2020 |
| Předmět: |
Cation binding
Aqueous solution Materials science Molecular logic gate 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Binding constant Atomic and Molecular Physics and Optics 0104 chemical sciences Spectrometry Fluorescence Fluorescence microscope Physical chemistry Humans General Materials Science Titration 0210 nano-technology Instrumentation Mulliken population analysis Spectroscopy Aluminum Fluorescent Dyes |
| Zdroj: | Methods and applications in fluorescence. 8(3) |
| ISSN: | 2050-6120 |
| Popis: | An easy to make organic probe (hereafter called as R) possessing multiple ligating sites have been synthesized and characterized using spectral techniques. The probe exhibits selective and sensitive turn-on fluorescence response with Al(III) in aqueous dimethylformamide (DMF) (1:1 v/v) solution. Fluorescence titration experiment shows that the probe binds with Al(III) with a 1:1 stoichiometry and a binding constant of 6.6 × 104 M-1.The mode of coordination of R with Al(III) has been established suing 27Al and 1H NMR studies and the results suggest formation of an octahedral complex been them. The suggested point of attachment of R with Al(III) corroborates well with Density Functional Theory (DFT) optimized structure and Mulliken charges computed. Chelation-enhanced fluorescence (CHEF) is proposed as the mechanism of enhancement of fluorescence upon addition of Al(III) to R. The probe detects Al(III) in aqueous solution with a detection limit of 0.2 μM, which is much lower than the permissible limit of Al(III) set by the World Health Organization (WHO).The probe works in a wide pH range (4-11) and thus makes it a suitable candidate for environmental and biological applications. The fluorescence signals of R were used to construct an INHIBIT molecular logic gate. The confocal fluorescence microscope experiments show that R could be employed as a fluorescent probe for detecting Al(III) in living cells. |
| Databáze: | OpenAIRE |
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