Type-specific radioimmunoassays for the gp70s of mink cell focus-inducing murine leukemia viruses: expression of a cross-reacting antigen in cells infected with the friend strain of the spleen focus-forming virus
| Autor: | David L. Linemeyer, John Feild, Edward M. Scolnick, Sandra K. Ruscetti, D H Troxler |
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| Jazyk: | angličtina |
| Rok vydání: | 1978 |
| Předmět: |
Genes
Viral viruses Immunology Radioimmunoassay Spleen Focus-Forming Virus Cross Reactions Recombinant virus Virus law.invention Cell Line Gene product Viral Proteins Antigen Species Specificity law biology.animal hemic and lymphatic diseases Immunology and Allergy Mink skin and connective tissue diseases Antigens Viral Glycoproteins Recombination Genetic biology Articles Virology Molecular biology Friend murine leukemia virus Cell culture Recombinant DNA |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | We have isolated the gp70 of a helper-independent strain of a Friend mink cell focus-inducing (MCF) virus, Fr-MCF-1. This recombinant virus, like the previously described AKR-MCF viruses, has been shown by both biological and biochemical means to be an envelope gene recombinant between Friend murine leukemia virus (F-MuLV) and a mouse xenotropic virus. Utilizing (125)I- labeled Fr-MCF-1 gp70 and antiserum prepared against an MCF strain of Moloney type-C virus (Mol-MCF(83)), we have developed a radioimmunoassay which detects immunological determinant (s)contained in the gp70s of MCF viruses derived from F-MuLV, Mol-MuLV, and AKR-MuLV. This MCF determinant(s) is not detected in the ecotropic parents of each of these MCF viruses, nor in helper-independent murine xenotropic viruses derived from Swiss or BALB/c mice. A protein partially cross-reactive with the MCF gp70 determinant(s) is detected in a replicating xenotropic virus derived from NZB mice. Utilizing this MCF gp70 specific immunoassay, we can detect a cross-reacting gene product coded for by the Friend strain of the spleen focus-forming virus (SFFV) in rat fibroblasts nonproductively infected with SFFV. The results support earlier molecular hybridization studies which indicated that the genome of SFFV contains genetic information derived from both F-MuLV and xenotropic virus, and that the xenotropic-related sequences in SFFV are highly related to those found in MCF murine type-C viruses. |
| Databáze: | OpenAIRE |
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