Charge Neutralization Strategy to Construct Salt-Tolerant and Cell-Permeable Nanoprobes: Application in Ratiometric Sensing and Imaging of Intracellular pH
| Autor: | Meiting Xie, Jiating Cao, Chao Lu, Jianguo Wang, Weijiang Guan, Zhuoyong Zhang, Wenjuan Zhou, Xinyu Gao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Diagnostic Imaging Chemistry Intracellular pH Salt (chemistry) Metal Nanoparticles Hydrogen-Ion Concentration Neutralization Analytical Chemistry Nanoclusters Pyranine chemistry.chemical_compound Spectrometry Fluorescence Biophysics Gold Nanocarriers Biosensor Intracellular Fluorescent Dyes |
| Zdroj: | Analytical chemistry. 93(45) |
| ISSN: | 1520-6882 |
| Popis: | Intracellular pH homeostasis is essential for the survival and function of biological cells. Negatively charged molecular probes, such as pyranine (HPTS), tend to exhibit poor salt tolerance and unsatisfactory cell permeability, limiting their widespread use in intracellular assays. Herein, we explored a charge neutralization strategy using multicharged cationic nanocarriers for an efficient and stable assembly with the pH-sensitive HPTS. Through immobilization and neutralization with poly(allylamine hydrochloride)-stabilized red-emitting gold nanoclusters (PAH-AuNCs), the resulting nanoprobes (HPTS-PAH-AuNCs) offered improved salt tolerance, satisfactory cell permeability, and dual-emission properties. The fluorescence ratio exhibited a linear response over the pH range of 3.0-9.0. Moreover, the proposed HPTS-PAH-AuNCs were successfully applied to determine and visualize lysosomal pH variations in living cells, which indicated great potential for biosensing and bioimaging applications in living systems. Benefiting from the charge neutralization strategy, various types of probes can be expected to achieve broader analytical applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|