Mutations in the predicted DNA polymerase subunit POLD3 result in more rapid flowering of Brachypodium distachyon
| Autor: | Leah Varner, Curtis G. Wilkerson, Kevin S. Mayer, Richard M. Amasino, Debbie Laudencia-Chingcuanco, Frédéric Bouché, Thomas S. Ream, John P. Vogel, Amy Cartwright, Richard Sibout, Daniel P. Woods, Yinxin Dong, Nicholas Thrower |
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| Přispěvatelé: | Laboratory of Genetics, University of Wisconsin-Madison, United States Department of Energy Great Lakes Bioenergy Research Center, Department of Biochemistry, College of Horticulture, Northwest A&F University, Department of Plant Biology and Department of Molecular Biology and Biochemistry, Michigan State University [East Lansing], Michigan State University System-Michigan State University System, United States Department of Energy Joint Genome Institute, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), USDA-ARS Western Regional Research Center, Lawrence Berkeley National Laboratory [Berkeley] (LBNL), University of California [Berkeley], University of California, National Science Foundation (NSF)IOS-1258126IOS-1755224United States Department of Energy (DOE)BER DE-FC02-07ER64494United States Department of Energy (DOE)DE-AC02-05CH11231FP000004794Wallonie Bruxelles international fellowship INRA (Institut de la Recherche Agronomique) Fonds de la Recherche Scientifique - FNRS |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology DNA polymerase Mutant POLD3 Plant Science Flowers medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Gene expression medicine Gene DNA Polymerase III Plant Proteins Genetics Mutation Brachypodium distachyon flowering biology Reproduction fungi food and beverages [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics biology.organism_classification Phenotype 030104 developmental biology grasses chemistry biology.protein DNA 010606 plant biology & botany Brachypodium |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2020, 227 (6), pp.1725-1735. ⟨10.1111/nph.16546⟩ |
| ISSN: | 0028-646X 1469-8137 |
| Popis: | The timing of reproduction is a critical developmental decision in the life cycle of many plant species.Fine mapping of a rapid-flowering mutant was done using whole-genome sequence data from bulked DNA from a segregating F2 mapping populations. The causative mutation maps to a gene orthologous with the third subunit of DNA polymerase delta (POLD3), a previously uncharacterized gene in plants. Expression analyses of POLD3 were conducted via real time qPCR to determine when and in what tissues the gene is expressed.To better understand the molecular basis of the rapid-flowering phenotype, transcriptomic analyses were conducted in the mutant vs wild-type. Consistent with the rapid-flowering mutant phenotype, a range of genes involved in floral induction and flower development are upregulated in the mutant.Our results provide the first characterization of the developmental and gene expression phenotypes that result from a lesion in POLD3 in plants. |
| Databáze: | OpenAIRE |
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