Inhibition of Proliferation of ECV304 Cells by a Disintegrin from Chinese Green Tree Viper
| Autor: | Jian-Rong Xu, Yao-Ping Han, Xiang-Yun Lu, Yan-Ping Zhang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Umbilical Veins
Disintegrins Molecular Sequence Data Size-exclusion chromatography Venom Viper Venoms Tripeptide Biochemistry Cell Line Structural Biology Complementary DNA Crotalid Venoms Disintegrin Animals Humans Trimeresurus Amino Acid Sequence Cloning Molecular Peptide sequence Cell Proliferation Base Sequence biology Cell growth General Medicine biology.organism_classification Molecular biology Trimeresurus stejnegeri biology.protein Endothelium Vascular Sequence Alignment Platelet Aggregation Inhibitors |
| Zdroj: | Protein & Peptide Letters. 14:437-441 |
| ISSN: | 0929-8665 |
| Popis: | A novel disintegrin, stejnin, was purified from the Trimeresurus stejnegeri venom by gel filtration and reverse phase high performance liquid chromatography. The molecular weight of stejnin was determined to be 7428 Da by MALD-TOF MS analysis. The cDNA encoding the precursor of stejnin was cloned from the venom gland. From the deduced amino acid sequence, stejnin is composed of 71 amino acid residues contains the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Stejnin strongly inhibited ADP- and ristomycin-induced human platelet aggregation with IC50 of 45 and 50 nM, respectively. Stejnin also possessed potent inhibited cell proliferation of ECV304 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|