Oxidative stress in hypertensive patients induces an increased contractility in vein grafts independent of endothelial function
| Autor: | Juan Muntaner, María Sofía Karbiner, Víctor Proto, Rodrigo O Maranon, Claudio M Joo Turoni, María Peral de Bruno |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Endothelial Dysfunction
medicine.medical_specialty Pathology lcsh:Diseases of the circulatory (Cardiovascular) system CIENCIAS MÉDICAS Y DE LA SALUD Antioxidant Article Subject medicine.medical_treatment Oxidative phosphorylation Fisiología medicine.disease_cause Nitric Oxide Nitric oxide Contractility chemistry.chemical_compound Internal medicine Internal Medicine medicine Endothelial dysfunction Vein business.industry Saphenous Veins Oxidative Stress purl.org/becyt/ford/3.1 [https] medicine.disease Medicina Básica Endocrinology medicine.anatomical_structure chemistry lcsh:RC666-701 Hypertension Immunohistochemistry purl.org/becyt/ford/3 [https] business Oxidative stress Research Article |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET International Journal of Hypertension, Vol 2011 (2011) International Journal of Hypertension |
| Popis: | Objective. To evaluate the impact of oxidative stress on vascular reactivity to vasoconstrictors and on nitric oxide (NO) bioavailability in saphenous vein (SV) graft with endothelial dysfunction from hypertensive patients (HT). Methods. Endothelial function, vascular reactivity, oxidative state, nitrites and NO release were studied in isolated SV rings from HT and normotensive patients (NT). Only rings with endothelial dysfunction were used. Results. HT rings presented a hyperreactivity to vasoconstrictors that was reverted by diphenylene iodonium (DPI). In NT, no effect of DPI was obtained, but Nω-nitro - L - arginine methyl ester (L-NAME) increased the contractile response. NO was present in SV rings without endothelial function. Nitrites were higher in NT than in HT (1066.1 ± 86.3 pmol/mg; n = 11 versus 487.8 ± 51.6; n = 23; P 0.01) and inhibited by nNOS inhibitor. L-arginine reversed this effect. Antioxidant agents increased nitrites and NO contents only in HT. The anti-nNOS-stained area by immunohistochemistry was higher in NT than HT. HT showed an elevation of oxidative state. Conclusions. Extraendothelial NO counter-regulates contractility in SV. However, this action could be altered in hypertensive situations by an increased oxidative stress or a decreased ability of nNOS to produce NO. Further studies should be performed to evaluate the implication of these results in graft patency rates. Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Karbiner, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Muntaner Juan. Centro Modelo de Cardiología S.r.l.; Argentina Fil: Proto, Victor. Centro Modelo de Cardiología S.r.l.; Argentina Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina |
| Databáze: | OpenAIRE |
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