Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity
| Autor: | Dominique Langin, Vladimir Stich, Michaela Vitkova, Nathalie Viguerie, Cedric Moro, Jiri Hanacek, Eva Klimcakova, Magda Bajzova, Jan Polak, Carine Valle, Frédéric Capel, Michaela Kovacikova, Blanka Richterova, Jindra Hejnova |
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| Přispěvatelé: | Simon, Marie Francoise, Hepatic and adipose tissue and functions in the metabolic syndrome - HEPADIP - 32591 - OLD, Franco-Czech Laboratory for Clinical Research on Obesity, Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Mother and Child Care, Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), The authors' work is supported by Institut National de la Sante' et de la Recherche Me'dicale, Grant GACR 303/04/0158 of the Grant Agency of the Czech Republic, research project of the Ministry of Education of Czech Republic MSM 0021620814, RIOMA project of the Agence Nationale de la Recherche and the project 'Hepatic and adipose tissue and functions in the metabolic syndrome' (HEPADIP, see http://www.hepadip.org/), which is supported by the European Commission as an Integrated Project under the 6th Framework Programe (Contract LSHM-CT-2005-018734), European Project: 32591,HEPADIP, Université de Toulouse (UT)-Université de Toulouse (UT)- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biochimie [Purpan], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse] |
| Rok vydání: | 2007 |
| Předmět: |
Endocrinology
Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry RNA Messenger/metabolism Adipose tissue Gene Expression Retinol-Binding Proteins/*genetics/*metabolism MESH: Glucose Clamp Technique Biochemistry Glucose Transporter Type 4/genetics 0302 clinical medicine Endocrinology Weight loss MESH: Obesity Insulin Gene Expression/physiology MESH: Diet Reducing 2. Zero hunger 0303 health sciences MESH: Middle Aged Glucose Transporter Type 4 Middle Aged Very low calorie diet MESH: Insulin Resistance Adipose Tissue Female medicine.symptom MESH: Retinol-Binding Proteins Plasma MESH: Adipose Tissue Adult medicine.medical_specialty MESH: Gene Expression Diet Reducing Diet therapy food.diet Calorie restriction 030209 endocrinology & metabolism MESH: Insulin Biology Obesity/*diet therapy/*physiopathology Caloric Restriction MESH: Weight Loss 03 medical and health sciences food Insulin resistance Internal medicine Weight Loss [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology medicine Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Obesity RNA Messenger MESH: RNA Messenger 030304 developmental biology MESH: Caloric Restriction Retinol binding protein 4 MESH: Humans Biochemistry (medical) Adipose Tissue/*physiology MESH: Adult MESH: Retinol-Binding Proteins medicine.disease Retinol-Binding Proteins biology.protein Glucose Clamp Technique Weight Loss/physiology MESH: Glucose Transporter Type 4 Insulin Resistance Insulin/blood MESH: Female Retinol-Binding Proteins Plasma |
| Zdroj: | Journal of Clinical Endocrinology and Metabolism Journal of Clinical Endocrinology and Metabolism, 2007, 92 (6), pp.2330-5. ⟨10.1210/jc.2006-2668⟩ Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2007, 92 (6), pp.2330-5. ⟨10.1210/jc.2006-2668⟩ |
| ISSN: | 0021-972X 1945-7197 |
| DOI: | 10.1210/jc.2006-2668⟩ |
| Popis: | 0021-972X (Print) 0021-972X (Linking) Journal Article Research Support, Non-U.S. Gov't; International audience; CONTEXT: Retinol-binding protein 4 (RBP4) may play a role in the development of insulin resistance. OBJECTIVE: We investigated whether RBP4 adipose tissue mRNA expression and plasma level are related to insulin sensitivity during a diet-induced weight loss. DESIGN, SETTING, PATIENTS, AND INTERVENTION: Obese women followed a dietary intervention composed of a 4-wk very low-calorie diet (VLCD), a 2-month low-calorie diet, and 3-4 months of a weight maintenance (WM) phase. MAIN OUTCOME MEASURES: Clinical investigation was performed before and at the end of each phase. Insulin sensitivity was assessed with the euglycemic hyperinsulinemic clamp. Adipose tissue mRNA and plasma levels of RBP4 were determined using reverse transcription-quantitative PCR and ELISA, respectively. RESULTS: Weight and fat mass decreased during VLCD and were stabilized during WM. Glucose disposal rate increased during VLCD and remained elevated thereafter. Plasma levels of RBP4 decreased after VLCD and, although increasing at subsequent phases, remained lower than prediet values. Adipose tissue mRNA levels were diminished after VLCD, and increased during low-calorie diet and WM to reach basal values. Basal RBP4 levels or diet-induced variations of RBP4 were not different in lean women and two groups of obese women with high- and low-insulin sensitivity. CONCLUSIONS: Severe calorie restriction promotes a reduction in adipose tissue and plasma levels of RBP4. The study does not bring evidence for a role for RBP4 in the regulation of diet-induced changes in insulin sensitivity. |
| Databáze: | OpenAIRE |
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