Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection

Autor: José de la Fuente, Luis Pedro Coelho, Lara Borges, Ana Domingos, Alejandro Cabezas-Cruz, Virgílio E. do Rosário, R. Pinheiro-Silva, James J. Valdés
Přispěvatelé: Fundação para a Ciência e a Tecnologia (Portugal), Instituto de Higiene e Medicina Tropical, Instituto de Medicina Molecular, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Biology Centre of the Czech Academy of Sciences (BIOLOGY CENTRE CAS), Czech Academy of Sciences [Prague] (CAS), Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University [Stillwater] (OSU), GHTM-UID/Multi/04413/2013 project, PhD course on Biomedical Sciences IHMT-UNL., Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Oklahoma State University [Stillwater], Instituto de Higiene e Medicina Tropical (IHMT), Global Health and Tropical Medicine (GHTM), Vector borne diseases and pathogens (VBD), Domingos, Ana, ProdInra, Migration
Rok vydání: 2015
Předmět:
Plasmodium berghei
[SDV]Life Sciences [q-bio]
health care facilities
manpower
and services
education
Glucose Transport Proteins
Facilitative
Receptors
Cell Surface
Salivary glands
Biology
Real-Time Polymerase Chain Reaction
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Mice
03 medical and health sciences
SDG 3 - Good Health and Well-being
RNA interference
Anopheles
parasitic diseases
Gene expression
Animals
Gene silencing
Gene Silencing
Gene
health care economics and organizations
Glucose transporter
030304 developmental biology
0303 health sciences
Gene knockdown
Sequence Analysis
RNA
Research
Gene Expression Profiling
Sporozoite
030302 biochemistry & molecular biology
biology.organism_classification
Virology
Anopheles coluzzii
3. Good health
[SDV] Life Sciences [q-bio]
RNA-seq
RNAi
Gene expression profiling
Infectious Diseases
Sialome
Host-Pathogen Interactions
Parasitology
Zdroj: Parasites & Vectors
Parasites & Vectors, 2015, 8 (1), ⟨10.1186/s13071-015-1079-8⟩
Parasites and Vectors
Parasites and Vectors, BioMed Central, 2015, 8 (1), ⟨10.1186/s13071-015-1079-8⟩
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Digital.CSIC. Repositorio Institucional del CSIC
instname
Parasites and Vectors 1 (8), . (2015)
ISSN: 1756-3305
Popis: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.-- et al.
[Background]: Malaria is a devastating infectious disease caused by Plasmodium parasites transmitted through the bites of infected Anopheles mosquitoes. Salivary glands are the only mosquito tissue invaded by Plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of Anopheles sialome highly relevant. [Methods]: RNA-sequencing was used to compare differential gene expression in salivary glands of uninfected and Plasmodium berghei-infected Anopheles coluzzii mosquitoes. RNA-seq results were validated by quantitative RT-PCR. The transmembrane glucose transporter gene AGAP007752 was selected for functional analysis by RNA interference. The effect of gene silencing on infection level was evaluated. The putative function and tertiary structure of the protein was assessed. [Results]: RNA-seq data showed that 2588 genes were differentially expressed in mosquitoes salivary glands in response to P. berghei infection, being 1578 upregulated and 1010 downregulated. Metabolism, Immunity, Replication/Transcription/Translation, Proteolysis and Transport were the mosquito gene functional classes more affected by parasite infection. Endopeptidase coding genes were the most abundant within the differentially expressed genes in infected salivary glands (P < 0.01)). Based on its putative function and expression level, the transmembrane glucose transporter gene, AGAP007752, was selected for functional analysis by RNA interference. The results demonstrated that the number of sporozoites was 44.3 % lower in mosquitoes fed on infected mice after AGAPP007752 gene knockdown when compared to control (P < 0.01)). [Conclusions]: Our hypothesis is that the protein encoded by the gene AGAPP007752 may play a role on An. coluzzii salivary glands infection by Plasmodium parasite, working as a sporozoite receptor and/or promoting a favorable environment for the capacity of sporozoites.
This work was partially supported by the GHTM-UID/Multi/04413/2013 project and by the PhD course on Biomedical Sciences, IHMT-UNL.
Databáze: OpenAIRE
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