Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in Brassica rapa L. toward identification of a novel self-incompatibility factor
| Autor: | Keita Suwabe, Kana Ito, Kohji Murase, Shunsuke Maeda, Satomi Sakazono, Masaaki Osaka, Sota Fujii, Masao Watanabe, Go Suzuki, Moe Nabemoto, Yong Pyo Lim, Hiromi Masuko-Suzuki, Yoshinobu Takada, Mikio Nakazono, Issei Kobayashi, Seiji Takayama |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Candidate gene Mutant Flowers Biology 010603 evolutionary biology 01 natural sciences Genetic analysis 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Brassica rapa Genetics Amino Acid Sequence Pollination Molecular Biology Gene 030304 developmental biology Glycoproteins Plant Proteins 0303 health sciences Sequence Analysis RNA food and beverages Chromosome General Medicine Plants Genetically Modified chemistry Haplotypes Genetic marker Organ Specificity Pollen Mutant Proteins Sequence Alignment DNA |
| Zdroj: | Genesgenetic systems. 94(4) |
| ISSN: | 1880-5779 |
| Popis: | Self-incompatibility (SI) is a sophisticated system for pollen selectivity to prevent pollination by genetically identical pollen. In Brassica, it is genetically controlled by a single, highly polymorphic S-locus, and the male and female S-determinant factors have been identified as S-locus protein 11 (SP11)/S-locus cysteine-rich protein (SCR) and S-locus receptor kinase (SRK), respectively. However, the overall molecular system and identity of factors in the downstream cascade of the SI reaction remain unclear. Previously, we identified a self-compatible B. rapa mutant line, TSC28, which has a disruption in an unidentified novel factor of the SI signaling cascade. Here, in a genetic analysis of TSC28, using an F2 population from a cross with the reference B. rapa SI line Chiifu-401, the causal gene was mapped to a genetic region of DNA containing markers BrSA64 and ACMP297 in B. rapa chromosome A1. By fine mapping using an F2 population of 1,034 plants, it was narrowed down to a genetic region between DNA markers ACMP297 and BrgMS4028, with physical length approximately 1.01 Mbp. In this genomic region, 113 genes are known to be located and, among these, we identified 55 genes that were expressed in the papilla cells. These are candidates for the gene responsible for the disruption of SI in TSC28. This list of candidate genes will contribute to the discovery of a novel downstream factor in the SP11-SRK signaling cascade in the Brassica SI system. |
| Databáze: | OpenAIRE |
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