Detection and identification of genetic material via single-molecule conductance
| Autor: | Sümeyye Gokce, Hashem Mohammad, Mashari Alangari, Büşra Demir, Joshua Hihath, Ersin Emre Oren, Yuanhui Li, M. P. Anantram, Juan M. Artés |
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| Přispěvatelé: | TOBB ETÜ, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümü, TOBB ETU, Faculty of Engineering, Department of Biomedical Engineering, Ören, Ersin Emre, LaserLaB - Energy, Biophysics Photosynthesis/Energy |
| Rok vydání: | 2018 |
| Předmět: |
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Molecular Biomedical Engineering Electrons Bioengineering Biosensing Techniques 02 engineering and technology Computational biology 010402 general chemistry medicine.disease_cause Polymorphism Single Nucleotide 01 natural sciences Bacterial genetics Electron Transport chemistry.chemical_compound Escherichia coli medicine Humans Molecule General Materials Science Electrical and Electronic Engineering Enhancer Escherichia coli Infections Base Sequence Chemistry Oligonucleotide Electric Conductivity Conductance RNA DNA 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics 0104 chemical sciences RNA Bacterial SDG 6 - Clean Water and Sanitation 0210 nano-technology |
| Zdroj: | Li, Y, Artés, J M, Demir, B, Gokce, S, Mohammad, H M, Alangari, M, Anantram, M P, Oren, E E & Hihath, J 2018, ' Detection and identification of genetic material via single-molecule conductance ', Nature Nanotechnology, vol. 13, no. 12, pp. 1167-1173 . https://doi.org/10.1038/s41565-018-0285-x Nature Nanotechnology, 13(12), 1167-1173. Nature Publishing Group |
| ISSN: | 1748-3395 1748-3387 |
| DOI: | 10.1038/s41565-018-0285-x |
| Popis: | The ongoing discoveries of RNA modalities (for example, non-coding, micro and enhancer) have resulted in an increased desire for detecting, sequencing and identifying RNA segments for applications in food safety, water and environmental protection, plant and animal pathology, clinical diagnosis and research, and bio-security. Here, we demonstrate that single-molecule conductance techniques can be used to extract biologically relevant information from short RNA oligonucleotides, that these measurements are sensitive to attomolar target concentrations, that they are capable of being multiplexed, and that they can detect targets of interest in the presence of other, possibly interfering, RNA sequences. We also demonstrate that the charge transport properties of RNA:DNA hybrids are sensitive to single-nucleotide polymorphisms, thus enabling differentiation between specific serotypes of Escherichia coli. Using a combination of spectroscopic and computational approaches, we determine that the conductance sensitivity primarily arises from the effects that the mutations have on the conformational structure of the molecules, rather than from the direct chemical substitutions. We believe that this approach can be further developed to make an electrically based sensor for diagnostic purposes. |
| Databáze: | OpenAIRE |
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