Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa
| Autor: | Maurycy Daroch, Andrew D. Bates, Catharine A. Houghton, Andrew J. Carnell, Mark C. Wilkinson, Jonathan K. Moore, Lesley A. Iwanejko |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Complementary Glycosylation Genes Fungal Molecular Sequence Data Bioengineering Peptide Context (language use) Applied Microbiology and Biotechnology Biochemistry Substrate Specificity Fungal Proteins Protein sequencing Complementary DNA Enzyme Stability Amino Acid Sequence Cloning Molecular DNA Fungal Gene Library Glycoproteins chemistry.chemical_classification Laccase Chromatography Base Sequence Sequence Homology Amino Acid biology Inverse polymerase chain reaction Stropharia aeruginosa RNA Fungal Sequence Analysis DNA biology.organism_classification Molecular biology Molecular Weight chemistry Pyrosequencing Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet Agaricales Protein Processing Post-Translational Sequence Alignment Biotechnology |
| Zdroj: | Enzyme and Microbial Technology. :1-7 |
| ISSN: | 0141-0229 |
| DOI: | 10.1016/j.enzmictec.2014.02.003 |
| Popis: | Here we describe the identification, purification and characterisation of glycosylated yellow laccase proteins from the basidiomycete fungus Stropharia aeruginosa. Biochemical characterisation of two yellow laccases, Yel1p and Yel3p, show that they are both secreted, monomeric, N-glycosylated proteins of molecular weight around 55kDa with substrate specificities typical of laccases, but lacking the absorption band at 612nm typical of the blue laccase proteins. Low coverage, high throughput 454 transcriptome sequencing in combination with inverse-PCR was used to identify cDNA sequences. One of the cDNA sequences has been assigned to the Yel1p protein on the basis of identity between the translated protein sequence and the peptide data from the purified protein, and the full length gene sequence has been obtained. Biochemical properties, substrate specificities and protein sequence data have been used to discuss the unusual spectroscopic properties of S. aeruginosa proteins in the context of recent theories about the differences between yellow and blue laccases. |
| Databáze: | OpenAIRE |
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