Characterization of red cell membrane proteins as a function of red cell density: Annexin VII in different forms of hereditary spherocytosis
| Autor: | Francesco Salvatore, Stefania Orrù, Gordon W. Stewart, Achille Iolascon, Luigi Del Vecchio, Silverio Perrotta, Marianna Caterino, Margherita Ruoppolo, Marcella Savoia |
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| Přispěvatelé: | Caterino, Marianna, Ruoppolo, Margherita, Orru, S, Savoia, Marcella, Perrotta, S, DEL VECCHIO, Luigi, Salvatore, Francesco, Stewart, Gw, Iolascon, Achille, Caterino, M, Ruoppolo, M, Savoia, M, Perrotta, Silverio, Vecchio, Ld, Salvatore, F, Iolascon, A. |
| Jazyk: | angličtina |
| Předmět: |
Ankyrins
Proteomics Erythrocytes Biophysics Cell Count Spherocytosis Hereditary Hereditary spherocytosis Biochemistry Annexin VII Hereditary spherocytosi Structural Biology Anion Exchange Protein 1 Erythrocyte Membrane proteins Genetics medicine Humans Ankyrin Annexin A7 Electrophoresis Gel Two-Dimensional Spectrin Molecular Biology Band 3 chemistry.chemical_classification biology Mass spectrometry Erythrocyte Membrane Erythrocyte Aging Cell Biology medicine.disease Molecular biology Red blood cell Protein Transport medicine.anatomical_structure Erythrocyte maturation chemistry Membrane protein biology.protein Percoll |
| Zdroj: | FEBS Letters. (28-29):6527-6532 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2006.10.070 |
| Popis: | Fresh human blood samples were collected from healthy controls and splenectomized and unsplenectomized patients with hereditary spherocytosis due to band 3 or ankyrin and spectrin deficiency. The erythrocytes were separated into age-related fractions using self-forming Percoll density gradients. Membrane proteins were analysed by 2D electrophoresis and identified by mass spectrometry.Annexin VII was present in reticulocytes but was then lost as the cells matured. A different pattern was found in band 3-deficient samples: annexin VII was in fact present in both mature and immature red cell membranes. Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation. |
| Databáze: | OpenAIRE |
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