Assessment of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification and biotyping of the pathogenic oomycete Pythium insidiosum
| Autor: | Yothin Kumsang, Atthapol Srimongkol, Paisan Jittorntam, Onrapak Reamtong, Theerapong Krajaejun, Tassanee Lohnoo, Wanta Yingyong, Suthee Mangmee, Thidarat Rujirawat |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Fusarium Genotyping Techniques 030106 microbiology Pythium Matrix assisted laser desorption ionization time of flight Mass spectrometry Pythium insidiosum DNA Ribosomal lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences Pythiosis Humans lcsh:RC109-216 Phylogeny Oomycete biology Sequence Analysis DNA General Medicine biology.organism_classification Matrix-assisted laser desorption/ionization 030104 developmental biology Infectious Diseases Class Zygomycetes Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | International Journal of Infectious Diseases, Vol 77, Iss, Pp 61-67 (2018) |
| ISSN: | 1201-9712 |
| Popis: | Objective: Pythiosis is a life-threatening infectious disease caused by the oomycete Pythium insidiosum. The disease has been increasingly reported worldwide. Most patients with pythiosis undergo surgical removal of an infected organ. Early diagnosis contributes to better prognosis of pythiosis patients. Here, we assessed the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and biotyping of P. insidiosum. Methods: A comprehensive set of mass spectra of P. insidiosum was generated to evaluate MALDI-TOF MS for identification and biotyping of P. insidiosum. Results: MALDI-TOF MS accurately identified all 13 P. insidiosum strains tested, at the species level. Mass spectra of P. insidiosum did not match any other microorganisms, including fungi (i.e., Aspergillus species, Fusarium species, and fungal species of the class Zygomycetes), which have similar microscopic morphologies with this oomycete. MALDI-TOF MS- and rDNA sequence-based biotyping methods consistently classified P. insidiosum into three groups: Clade-I (American strains), II (Asian and Australian strains), and III (mostly Thai strains). Conclusions: MALDI-TOF MS has been successfully used for identification and biotyping of P. insidiosum. The obtained mass spectral database allows clinical microbiology laboratories, well-equipped with a MALDI-TOF mass spectrometer, to conveniently identify P. insidiosum, without requiring any pathogen-specific reagents (i.e., antigen, antibody or primers). Keywords: Pythium insidiosum, Pythiosis, MALDI-TOF MS, Mass spectrum, Identification, Biotyping |
| Databáze: | OpenAIRE |
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