Proton Affinitive Derivatization for Highly Sensitive Determination of Testosterone and Dihydrotestosterone in Saliva Samples by LC-ESI-MS/MS
| Autor: | Ken-Ichi Ohno, Tomomi Tamura, Haruka Utsumi, Tomomi Hasegawa, Kouwa Yamashita |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Saliva medicine.medical_treatment Clinical Chemistry Tests Ion suppression in liquid chromatography–mass spectrometry Picolinic acid 01 natural sciences Analytical Chemistry Steroid 03 medical and health sciences chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry medicine Humans Testosterone Derivatization Chromatography Chemistry Elution 010401 analytical chemistry Dihydrotestosterone 0104 chemical sciences 030104 developmental biology Protons Chromatography Liquid medicine.drug |
| Zdroj: | Analytical Sciences. 34:1017-1021 |
| ISSN: | 1348-2246 0910-6340 |
| Popis: | In this study, proton affinitive derivatization using picolinic acid and its analogs (3- and 6-methylpicolinic acid and 5-butylpicolinic acid) with proton affinitive moieties was performed for the highly sensitive determination of testosterone (T) and 5α-dihydrotestosterone (DHT) in saliva by LC-ESI-MS/MS. T and DHT were converted to their corresponding picolinate esters and their chromatographic behavior was investigated with a reversed phase column. The picolinate ester of each steroid exhibited a clear single peak and elution occurred in the following order: picolinate, 3/6-methylpicolinate, and 5-butylpicolinate. Estimation and understanding of the separation and retention time of each picolinate ester was made simple using the develop method. Although the peaks of picolinate and 3/6-methylpicolinate esters were suppressed by interference from the saliva background (matrix effect), the 5-butylpicolinate esters were only marginally affected. |
| Databáze: | OpenAIRE |
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