Evaluation of parallel operated small-scale bubble columns for microbial process development using Staphylococcus carnosus
| Autor: | S. Dilsen, A. Sandgathe, D. Herforth, C. Wandrey, W. Paul, Jutta Altenbach-Rehm, Dirk Weuster-Botz, Roland Freudl |
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| Rok vydání: | 2001 |
| Předmět: |
Glycerol
Chromatography biology Chemistry Bubble Recombinant Fusion Proteins Staphylococcus chemistry.chemical_element Bioengineering General Medicine Hydrogen-Ion Concentration equipment and supplies biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Industrial Microbiology Dry weight Batch processing Yeast extract Energy source Carbon Cell Division Biotechnology Staphylococcus carnosus |
| Zdroj: | Journal of biotechnology. 88(1) |
| ISSN: | 0168-1656 |
| Popis: | Shake flasks and pH-controlled small-scale bubble columns were compared with respect to their usefulness as a basic tool for process development for human calcitonin precursor fusion-protein production with Staphylococcus carnosus . Parallel control of the pH (and making use of the base addition data) is necessary to study the effects of medium composition, to identify pH-optima and to develop a medium, which minimizes the acid excretion of S. carnosus . This medium with glycerol as energy source and yeast extract as carbon and nitrogen source resulted in cell dry weight concentration in shake flasks of 5 g l −1 , which were thus improved by a factor of 10. Cell dry weight concentrations of up to 12.5 g l −1 were measured in the batch process with pH-controlled small-scale bubble columns due to their higher oxygen transfer capability. In contrast to shake flasks it was demonstrated, that the batch process performance of recombinant S. carnosus secreting the human calcitonin precursor fusion-protein was identical within the estimation error in pH-controlled small-scale bubble columns compared to the stirred-tank reactor. |
| Databáze: | OpenAIRE |
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