Expression of active recombinant human alpha 1-antitrypsin in transgenic rabbits
| Autor: | Wilfried Dalemans, Marie-Georges Stinnakre, Huguette Schultz, Rainer Bischoff, Hervé Pointu, Daniel Clesse, Joe Attal, M. Massoud, Andrea Pavirani, Louis-Marie Houdebine |
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| Přispěvatelé: | Rijksuniversiteit Groningen, Synthesis and Analysis, Unité de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | Dutch; Flemish |
| Rok vydání: | 1991 |
| Předmět: |
Microinjections
[SDV]Life Sciences [q-bio] Transgene Bioengineering Biology Applied Microbiology and Biotechnology law.invention Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine law Gene expression Animals Humans Gene Microinjection ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Pancreatic Elastase Pronucleus DNA General Medicine Blotting Northern Embryo Transfer Molecular biology Recombinant Proteins Genetically modified organism [SDV] Life Sciences [q-bio] Blot alpha 1-Antitrypsin 030220 oncology & carcinogenesis Recombinant DNA Female Rabbits Biotechnology |
| Zdroj: | J.Biotechnol., 18(3), 193-203 Journal of Biotechnology Journal of Biotechnology, Elsevier, 1991, 18, pp.193-204 |
| ISSN: | 0168-1656 |
| Popis: | A DNA construct containing the human alpha 1-antitrypsin gene including 1.5 and 4 kb of 5' and 3' flanking sequences, was microinjected into the pronucleus of rabbit embryos. The recombinant human protein was (a) expressed in the blood circulation of F0 and F1 transgenic rabbits at an average concentration of 1 mg ml-1, (b) shown to be fully active and (c) shown to be separable from its rabbit counterpart. Transgenic rabbits might represent a novel source of human proteins of therapeutic interest. |
| Databáze: | OpenAIRE |
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