Prostacyclin administration suppresses the increase in hepatic levels of COL1A(I) and glyceraldehyde-3-phosphate dehydrogenase mRNAs in the rat treated with carbon tetrachloride
| Autor: | Akiharu Watanabe, Hiroyuki Itoh, H Koyata, Koichi Hiraga, Terumi Takahara |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Biophysics Prostacyclin CCL4 Dehydrogenase Biology Biochemistry chemistry.chemical_compound Internal medicine medicine Animals RNA Messenger Carbon Tetrachloride Molecular Biology Glyceraldehyde 3-phosphate dehydrogenase Messenger RNA Carbon Tetrachloride Poisoning Glyceraldehyde-3-Phosphate Dehydrogenases Rats Inbred Strains Cell Biology Rats Kinetics medicine.anatomical_structure Endocrinology Liver chemistry Hepatocyte Carbon tetrachloride biology.protein Hepatic stellate cell Collagen medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 185:981-986 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)91723-4 |
| Popis: | Northern analysis using total RNAs from the component cells of normal rat liver indicated that COL1A(I) mRNA is present in fat-storing cells (Ito cells) and sinusoidal endothelial cells. A fraction for Kupffer cells also contained this mRNA. When CCl4 was given, COL1A(I) mRNA was increased in a factor of 1.5 in the fractions of these component cells. After 48 h of the drug administration, hepatocytes appeared to possess over 60% of liver COL1A(I) mRNA, although in normal hepatocytes its level was below the range detectable by our procedures. Under this injured condition of liver, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA level was elevated, while activity of this enzyme was lowered by 50% of the control value. All the changes were obviously suppressed by the simultaneous administration of prostacyclin. |
| Databáze: | OpenAIRE |
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