Stimulation of choline acetyltransferase activity by ethanol in in vitro preparations of rat cerebrum
| Autor: | Ruth Berman Reisberg |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Male
Time Factors Stimulation Alcohol Sodium Chloride General Biochemistry Genetics and Molecular Biology Choline chemistry.chemical_compound Acetyltransferases medicine Animals Carbon Radioisotopes General Pharmacology Toxicology and Pharmaceutics Incubation chemistry.chemical_classification Ethanol Chromatography Chemistry Cerebrum Brain General Medicine Chromatography Ion Exchange In vitro Rats Kinetics Enzyme medicine.anatomical_structure Biochemistry Ultracentrifugation Choline chloride |
| Zdroj: | Life sciences. 14(10) |
| ISSN: | 0024-3205 |
| Popis: | Choline acetyltransferase activity in homogenates, or in partially purified extracts of rat brain cerebra, was increased by 11–37% in the presence of ethanol when incubated at 38°C with [ 14 C] acetyl-CoA, choline chloride and alcohol concentrations of 0.17M to 1.02M. In preincubation experiments with enzyme preparations and ethanol, inactivation of the enzyme by the alcohol, which occurs at incubation times longer than 20 minutes, could be at least partially prevented by the addition of certain components of the incubation mixture to the preincubation mixture. |
| Databáze: | OpenAIRE |
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