Cytotoxic T lymphocyte responses to a liposome-adjuvanted influenza A virus vaccine in the elderly
| Autor: | Mary C. Manning, Patricia J Pietrobon, Peter J. Hanscome, Douglas C. Powers |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cellular immunity
Time Factors viruses Orthomyxoviridae medicine.disease_cause Virus Adjuvants Immunologic Double-Blind Method Influenza Human Influenza A virus Immunology and Allergy Cytotoxic T cell Medicine Humans Aged Aged 80 and over Dosage Forms biology business.industry biology.organism_classification Virology Vaccination CTL Infectious Diseases Immunization Influenza Vaccines Immunology Liposomes business T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of infectious diseases. 172(4) |
| ISSN: | 0022-1899 |
| Popis: | This randomized double-blind study evaluated cytotoxic T lymphocyte (CTL) responses of elderly volunteers after parenteral immunization with either liposome-adjuvanted (n = 23) or control subvirion (n = 26) vaccine containing detergent-split influenza A/Taiwan/1/86 (H1N1) virus. Peripheral blood mononuclear cells obtained 0, 2, and 12 weeks after vaccination were stimulated in vitro with influenza A (H1N1) virus-infected autologous cells and then assayed for influenza virus-specific cytotoxicity using autologous virus-infected target cells. CTL responses to vaccination exhibited influenza A virus heterosubtypic cross-reactivity and were mediated primarily by CD8 + effector cells. Anti-influenza virus CTL activity was enhanced to a significantly greater extent by the liposome vaccine than by the control subvirion vaccine. It remains to be established whether the advantage of a liposomal formulation in terms of an improved CTL response is relevant to vaccine protective efficacy. |
| Databáze: | OpenAIRE |
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